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Severity of Acute Infectious Mononucleosis Correlates with Cross-Reactive Influenza CD8 T-Cell Receptor Repertoires Nuray Aslan et al 2017

Discussion in 'Other health news and research' started by Dolphin, Dec 7, 2017.

  1. Dolphin

    Dolphin Senior Member (Voting Rights)


    Severity of Acute Infectious Mononucleosis Correlates with Cross-Reactive Influenza CD8 T-Cell Receptor Repertoires

    1. Nuray Aslan a,
    2. Levi B. Watkin a,
    3. Anna Gil a,
    4. Rabinarayan Mishra a,
    5. Fransenio G. Clarka,d,
    6. Raymond M. Welsh a,
    7. Dario Ghersi b,
    8. Katherine Luzuriaga c,d,
    9. Liisa K. Selin a,d
    1. Jack R. Bennink, Editor
    +Author Affiliations

    1. liisa.selin@umassmed.edu.

    Fifty years after the discovery of Epstein-Barr virus (EBV), it remains unclear how primary infection with this virus leads to massive CD8 T-cell expansion and acute infectious mononucleosis (AIM) in young adults.

    AIM can vary greatly in severity, from a mild transient influenza-like illness to a prolonged severe syndrome.

    We questioned whether expansion of a unique HLA-A2.01-restricted, cross-reactive CD8 T-cell response between influenza virus A-M158 (IAV-M1) and EBV BMLF1280(EBV-BM) could modulate the immune response to EBV and play a role in determining the severity of AIM in 32 college students.

    Only ex vivo total IAV-M1 and IAV-M1+EBV-BM cross-reactive tetramer+ frequencies directly correlated with AIM severity and were predictive of severe disease.

    Expansion of specific cross-reactive memory IAV-M1 T-cell receptor (TCR) Vβ repertoires correlated with levels of disease severity.

    There were unique profiles of qualitatively different functional responses in the cross-reactive and EBV-specific CD8 T-cell responses in each of the three groups studied, severe-AIM patients, mild-AIM patients, and seropositive persistently EBV-infected healthy donors, that may result from differences in TCR repertoire use. I

    AV-M1 tetramer+ cells were functionally cross-reactive in short-term cultures, were associated with the highest disease severity in AIM, and displayed enhanced production of gamma interferon, a cytokine that greatly amplifies immune responses, thus frequently contributing to induction of immunopathology.

    Altogether, these data link heterologous immunity via CD8 T-cell cross-reactivity to CD8 T-cell repertoire selection, function, and resultant disease severity in a common and important human infection.

    In particular, it highlights for the first time a direct link between the TCR repertoire with pathogenesis and the diversity of outcomes upon pathogen encounter.


    The pathogenic impact of immune responses that by chance cross-react to unrelated viruses has not been established in human infections.

    Here, we demonstrate that the severity of acute infectious mononucleosis (AIM), an Epstein-Barr virus (EBV)-induced disease prevalent in young adults but not children, is associated with increased frequencies of T cells cross-reactive to EBV and the commonly acquired influenza A virus (IAV).

    The T-cell receptor (TCR) repertoire and functions of these cross-reactive T cells differed between mild- and severe-AIM patients, most likely because these two groups of patients had selected different memory TCR repertoires in response to IAV infections encountered earlier.

    This heterologous immunity may explain variability in disease outcome and why young adults with more-developed IAV-specific memory T-cell pools have more-severe disease than children, who have less-developed memory pools.

    This study provides a new framework for understanding the role of heterologous immunity in human health and disease and highlights an important developing field examining the role of T-cell repertoires in the mediation of immunopathology.

    • Citation Aslan N, Watkin LB, Gil A, Mishra R, Clark FG, Welsh RM, Ghersi D, Luzuriaga K, Selin LK. 2017. Severity of acute infectious mononucleosis correlates with cross-reactive influenza CD8 T-cell receptor repertoires. mBio 8:e01841-17. https://doi.org/10.1128/mBio.01841-17.
    Last edited by a moderator: Mar 19, 2021
  2. Dolphin

    Dolphin Senior Member (Voting Rights)

    Researchers connect severity of 'kissing disease' to T-cell population
    December 5, 2017

    American Society for Microbiology

    Acute infectious mononucleosis (AIM), also known as mono or the 'kissing disease,' is caused by the Epstein-Barr Virus (EBV). In a new paper, researchers connect the onset and severity of mono to T-cells that react to both EBV and the influenza A virus, which causes the flu. The study represents one of the first reported links between how a person's immune system responds to infection and receptors on T-cells, which instigate the immune response.

    continues: www.sciencedaily.com/releases/2017/12/171205104219.htm
  3. Andy

    Andy Committee Member

    Hampshire, UK
    Bumping this thread for this study, especially as two of the authors, Selin and Gil, have just been awarded a grant by Solve, see https://www.s4me.info/threads/solve-me-cfs-initiative-ramsay-grant-program-2019-awards.11952/
  4. Hutan

    Hutan Moderator Staff Member

    Aotearoa New Zealand
  5. Andy

    Andy Committee Member

    Hampshire, UK
    It occurs to me that this
    seems to hint at providing an explanation for the theorised "series of immune challenges" mechanism that has been talked about for ME. Obviously much work would need to be done to find out if it is an explanation or not.

    Or I'm reading too much into it... :)
  6. Susan K

    Susan K Established Member

    Boston, MA
    I really like what you are saying! Selin is onto something here. Yes she is a long-time T cell expert, and she's understated. Those are often the ppl who do important work.
  7. cassava7

    cassava7 Senior Member (Voting Rights)

    @Chris Ponting Have you seen this paper by chance? These findings from Selin and Gil's group seem very relevant to your presentation at CMRC2020 on T cell clonality as a potential biomarker for ME/CFS. The research study abstract for their Solve ME Ramsay award can be found here: https://solvecfs.org/liisa-selin-and-anna-gil/

    Makes me wonder how much progress Selin and Gil have quietly made so far... :)
    Last edited: Apr 18, 2020
    shak8, Ravn and Hutan like this.
  8. Chris Ponting

    Chris Ponting Established Member (Voting Rights)

    Thanks. I don't think this was replicated by Jackie Cliff et al. : https://www.frontiersin.org/articles/10.3389/fimmu.2019.00796/full
    Hutan, Ravn, Invisible Woman and 2 others like this.
  9. Trish

    Trish Moderator Staff Member

    Last edited: Mar 26, 2021
    Hutan, MEMarge and Andy like this.
  10. Invisible Woman

    Invisible Woman Senior Member (Voting Rights)

    Hutan likes this.
  11. Trish

    Trish Moderator Staff Member

    Oops, sorry, I've corrected it.
    Hutan, Invisible Woman and Mithriel like this.

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