Blog: 'Summary so far of "Something in the blood"' by Simon McGrath

The heat shock proteins help fold proteins,I think I saw some older studies saying those with M.E were low in heat shock proteins

 

Add another "Something in the blood" experiment from Alain Moreau. In his presentation at the Stanford Symposium Alain Moreau described using Jurkat cells (Jurkat cells are an immortalized line of human T lymphocyte cells, I'm guessing you purchase them from a supplier???) with healthy and ME/CFS plasma and measured the cellular impedance using a CellKey instrument at different plasma concentrations. Higher concentrations of ME/CFS plasma caused a higher increase in cellular impedance. [Graphs are Control, ME, Scoliosis]

It wasn't clear to me what patient subgroup the above chart is representative of.

He did show this response for all 5 subgroups which shows a lot of variation. This is the full slide


The bottom right shows all 5 subgroups + CTRL. Seems quite messy, so not sure what it is showing. Cellular impedance is very much dependant on how the cells touch the sensors on the plate, and how many touch........ leads to lots of questions.

He then showed how Jurkat cells soaked in patient plasma by subgroup for 2 hours responded differently to agonists


So to summarize he used a Jurkat cell line so all cells are from the same source and then mixed with patient plasma to produce a different cellular impedance response on his CellKey analyzer depending on plasma concentration and agonist applied.

{I hope I got that right)

 

For those interested in this section of Moreau's talk, you can go straight to it by copy-and-pasting this URL

Code:

https://www.facebook.com/OpenMedicineFoundation/videos/487148525184381/?t=8243

 

This thread ends in 2019. Have any of these findings been replicated?