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Protective Effect of Hemin Against Experimental Chronic Fatigue Syndrome in Mice: Possible Role of Neurotransmitters, 2020, Kumar et al

Discussion in 'BioMedical ME/CFS Research' started by Andy, Jun 9, 2020.

  1. Andy

    Andy Committee Member (& Outreach when energy allows)

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    Mouse model = mice they have exhausted through over exercising. Therefore this is of dubious use to us.
    Paywall, https://link.springer.com/article/10.1007/s12640-020-00231-y
    Open access PDF paper, https://sci-hub.tw/10.1007/s12640-020-00231-y
     
    Milo, Michelle, Simon M and 3 others like this.
  2. Snow Leopard

    Snow Leopard Senior Member (Voting Rights)

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    Indeed, torturing mice with forced swims might cause fatigue, but it certainly isn't CFS or ME.

    Side note: Heme oxygenase is HSP32. Heme is broken down into biliverdin (ultimately bilirubin) and Fe2+
    Gilbert's syndrome is not uncommon in CFS patients, suggesting this pathway has increased activity in many patients...
     
    Sarah94, alktipping, Michelle and 7 others like this.
  3. Sarah94

    Sarah94 Senior Member (Voting Rights)

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    Those poor mice :(
     
    alktipping, Michelle, rvallee and 3 others like this.
  4. mariovitali

    mariovitali Senior Member (Voting Rights)

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    This is very interesting ( cc : @wigglethemouse ), Thank you @Andy


    Upon searching for hemin i found that hemin is a TSPO ligand :



    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5520880/



    TSPO has been identified by Machine Learning since 2018. To be fair, there was no connection of its function with mitochondrial Calcium metabolism but rather with Cholesterol metabolism. Recall that Prusty also discussed about dysregulated calcium metabolism in one of his slides in his latest presentation.

    So, it could be of interest.
     
  5. adambeyoncelowe

    adambeyoncelowe Senior Member (Voting Rights)

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    This isn't ME. It's PTSD. It's horrific. How would the researchers like being 'forced to swim' (i.e., threatened with drowning) for 10 minutes per day?
     
    Snowdrop, Sarah94, alktipping and 4 others like this.
  6. rvallee

    rvallee Senior Member (Voting Rights)

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    This is about as valid and ethical as experiments where someone takes participants into a room and tell them that their parents were gruesomely murdered and use that state as a proxy for depression.

    Which, in mice, would be quite a feat.

    But, no, ME, or even CFS, is not the same as a healthy person (or mouse) exercising excessively. The big tell is right there in the name. Chronic. Chro-nic. Words. Mean. Things.
     
  7. Cytokinda

    Cytokinda Established Member

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    @mariovitali Hi Mario, Themos pointed me here after a twitter discussion of TSPO (also with @wigglethemouse)

    I find TSPO very interesting for its link to both mitochondrial enzymes and neuroinflammation. The connection to hemin is further intriguing for a number of reasons. I'll be brief here since TSPO perhaps deserves it's own separate thread (if there isn't one already on this site. I don't see one).

    I wonder if there are any (tenuous) lines to be drawn between hemin & some of the OMF blood studies, i.e. the RBC deformability study and the "something in healthy serum restores ME-cellular function under stress"-study. It seems too much to hope that hemin is this key factor but it's maybe worth consideration. I'd like to do some digging on how hemin arises endogenously in the body.



    Regarding the primary topic of this thread - yeah, what an atrocious mouse study. Mice studies tend to be that way, sadly.
     
    Andy and wigglethemouse like this.
  8. Mithriel

    Mithriel Senior Member (Voting Rights)

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    Basic biology is very conserved in mammals (and all eukaryotes) so research which tells us how normal biological functions work could lead to benefits finding what is wrong in ME, but animal models of disease are not very useful nowadays.

    They worked out what hormones are and how they function in animals and then used that knowledge to treat humans with endocrine diseases saving millions of lives but they did not start by trying to feed sugar into mice to give them diabetes.
     
    DigitalDrifter likes this.

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