1. Guest, click here, to read the 'News In Brief' post for w/c 15th October.
    Dismiss Notice

Pharmacological activation of AMPK and glucose uptake in cultured human skeletal muscle cells from patients with ME/CFS (2018) Newton et al

Discussion in 'BioMedical ME/CFS Research' started by hixxy, Apr 16, 2018.

  1. hixxy

    hixxy Established Member (Voting Rights)

    Messages:
    82
    Likes Received:
    514
    https://www.ncbi.nlm.nih.gov/pubmed/29654166
     
  2. Lisa108

    Lisa108 Senior Member (Voting Rights)

    Messages:
    174
    Likes Received:
    1,257
    Location:
    Germany
    A pdf of the accepted manuscript (not the final version) is here
     
  3. Amw66

    Amw66 Senior Member (Voting Rights)

    Messages:
    1,068
    Likes Received:
    6,376
    Interesting- potential upstream modulation paths mentioned ( including liver which has always been my hunch as part of the issue with my aunt and daughter).

    There were a few very interesting threads involving AMPK' s potential role in the other place.
     
  4. Trish

    Trish Moderator Staff Member

    Messages:
    7,157
    Likes Received:
    42,070
    Location:
    UK
    I have no idea what AMPK is or does. I googled and found this incredibly complicated diagram. My head exploded.

    https://media.cellsignal.com/www/pdfs/science/pathways/AMPK.pdf

    This was a little more helpful:
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3249400/
     
  5. strategist

    strategist Senior Member (Voting Rights)

    Messages:
    846
    Likes Received:
    8,616
    I keep saying there is an issue with glucose. Maybe I'm actually right :D
     
  6. Andy

    Andy Committee Member

    Messages:
    3,852
    Likes Received:
    28,955
    Location:
    Hampshire, UK
    Fukuda selection criteria with no mention of PEM again. I appreciate Newton for plugging away in the UK with biomedical studies but, in my opinion, she needs to change the criteria she uses.
     
  7. Amw66

    Amw66 Senior Member (Voting Rights)

    Messages:
    1,068
    Likes Received:
    6,376
    AMPK is also involved in " clock" processes, and could be significant in Naviaux's hypometabolic state hypothesis....
     
  8. adambeyoncelowe

    adambeyoncelowe Senior Member (Voting Rights)

    Messages:
    1,118
    Likes Received:
    7,608
    So much of this chimes with me.
     
  9. Amw66

    Amw66 Senior Member (Voting Rights)

    Messages:
    1,068
    Likes Received:
    6,376
  10. Estherbot

    Estherbot Established Member (Voting Rights)

    Messages:
    63
    Likes Received:
    559
  11. Estherbot

    Estherbot Established Member (Voting Rights)

    Messages:
    63
    Likes Received:
    559
    There's also a thread from AndyPandy who talks about their experiences withMetformin as a type 2 diabetic. They may be very different from people without Type 2 diabetes.

    ME and Metformin

    http://forums.phoenixrising.me/index.php?threads/me-and-metformin.47425/
     
    Last edited: Apr 16, 2018
  12. Estherbot

    Estherbot Established Member (Voting Rights)

    Messages:
    63
    Likes Received:
    559
    There's always a race to say this. ;)
     
  13. Andy

    Andy Committee Member

    Messages:
    3,852
    Likes Received:
    28,955
    Location:
    Hampshire, UK
    Is there? I wasn't aware of that.
     
  14. Estherbot

    Estherbot Established Member (Voting Rights)

    Messages:
    63
    Likes Received:
    559
    How the study was funded

    "Acknowledgements The work was funded by ME Research UK and supported by the NIHR Newcastle Clinical Research Facility."
     
  15. Estherbot

    Estherbot Established Member (Voting Rights)

    Messages:
    63
    Likes Received:
    559
    From the discussion

    "The important findings from this study are that, firstly, pharmacological activation of AMPK can improve glucose uptake in skeletal muscle cell cultures from patients with ME/CFS and secondly, cellular ATP content is significantly reduced in ME/CFS muscle cell cultures.

    The observation that AMPK was activated directly by metformin and 991 but not EPS (electrical pulse stimulation) in the ME/CFS cultures points to a signalling defect proximal to AMPK"
     
  16. Estherbot

    Estherbot Established Member (Voting Rights)

    Messages:
    63
    Likes Received:
    559
    Like @Trish, I've been trying to understand AMPK signalling without causing instant brain fog. Found this video on YouTube, which has enlightened me slightly.

    Out of many things AMPK does, it instructs cells to take in more glucose to make more ATP and to store less glucose as glycogen (in the liver) when the ratio of ATP drops.

    You can see the point if the AMPK signalling is messed up, it could cause energy shortages. (my non bio-chemist take)

    I'll shut up now & let the proper experts correct me and take over. @Simon M is a proper biochemist not a 5 minute one :)

     
    Inara, Hutan, Luther Blissett and 6 others like this.
  17. Jenny TipsforME

    Jenny TipsforME Senior Member (Voting Rights)

    Messages:
    346
    Likes Received:
    1,772
    I found some AMPK activators for a blog post 3 years ago



    I’ve been thinking about my very fluctuating ME journey over the last 20 years. I’ve been around 10% and around 95% on the Bell Disability Scale and everything in between up and down at different times. This week I’ve been thinking about when I went from 10% to 30% in a couple of weeks (with the help of a nutritionist). It could be a number of things but I started this supplement (Fast & be clear) at that point

    [​IMG]

    My understanding is Quercetin, alpha lipoic acid, grapes and green tea are AMPK activators. I wonder if this was part of the benefit of taking this supplement?
    http://ampkactivator.net/quercetin/
    http://ampkactivator.net/a-lipoic-acid/
     
  18. WillowJ

    WillowJ Senior Member (Voting Rights)

    Messages:
    172
    Likes Received:
    924
    That's exactly why I think the argument that doctors should already have a test for everything important is extremely silly and ignorant. Because metabolism is extraordinarily complex, and even all the complicated stuff that's known is not all of it yet.
     
  19. Amw66

    Amw66 Senior Member (Voting Rights)

    Messages:
    1,068
    Likes Received:
    6,376
    Cellular ATP content bring low is what Myhill, Booth et al found, together with low cellular magnesium bound ATP ( not active without Mg)
     
  20. Amw66

    Amw66 Senior Member (Voting Rights)

    Messages:
    1,068
    Likes Received:
    6,376
    I hate my phone - the keypad numbers are too small!!!!
     

Share This Page