Neat1 lncRNA organizes the inflammatory gene expressions in the dorsal root ganglion in neuropathic pain caused by nerve injury, 2023, Maruyama et al.

Discussion in 'Other health news and research' started by SNT Gatchaman, Aug 8, 2023.

  1. SNT Gatchaman

    SNT Gatchaman Senior Member (Voting Rights)

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    Neat1 lncRNA organizes the inflammatory gene expressions in the dorsal root ganglion in neuropathic pain caused by nerve injury
    Maruyama, Motoyo; Sakai, Atsushi; Fukunaga, Tsukasa; Miyagawa, Yoshitaka; Okada, Takashi; Hamada, Michiaki; Suzuki, Hidenori

    Primary sensory neurons regulate inflammatory processes in innervated regions through neuro-immune communication. However, how their immune-modulating functions are regulated in concert remains largely unknown.

    Here, we show that Neat1 long non-coding RNA (lncRNA) organizes the proinflammatory gene expressions in the dorsal root ganglion (DRG) in chronic intractable neuropathic pain in rats. Neat1 was abundantly expressed in the DRG and was upregulated after peripheral nerve injury. Neat1 overexpression in primary sensory neurons caused mechanical and thermal hypersensitivity, whereas its knockdown alleviated neuropathic pain.

    Bioinformatics analysis of comprehensive transcriptome changes indicated the inflammatory response was the most relevant function of genes upregulated through Neat1. Consistent with this, upregulation of proinflammatory genes in the DRG following nerve injury was suppressed by Neat1 knockdown. Expression changes of these proinflammatory genes were regulated through Neat1-mRNA interaction-dependent and -independent mechanisms. Notably, Neat1 increased proinflammatory genes by stabilizing its interacting mRNAs in neuropathic pain. Finally, Neat1 in primary sensory neurons contributed to spinal inflammatory processes that mediated peripheral neuropathic pain.

    These findings demonstrate that Neat1 lncRNA is a key regulator of neuro-immune communication in neuropathic pain.

    Link | PDF (Frontiers in Immunology)
     
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  2. SNT Gatchaman

    SNT Gatchaman Senior Member (Voting Rights)

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  3. Hutan

    Hutan Moderator Staff Member

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    I'm just about done for the day, but NEAT1 looks interesting.

    See also
    The lncRNA Neat1 promotes activation of inflammasomes in macrophages, 2019
    Excerpts from the 2019 paper below:
     
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  4. Creekside

    Creekside Senior Member (Voting Rights)

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    More complexity in how out bodies work. The cause of ME will be hard to find if it's due to some obscure molecule that is just the right shape to temporarily bond to a microRNA and thus affect its rate of function. I imagine that in some factory somewhere, parts flowed freely through a chute, but then a small change in the shape of the parts caused them to occasionally lock together in clumps, blocking the chute. Molecular shape is important in biochemistry too.
     

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