DMissa
Senior Member (Voting Rights)
There is a lot of talk in this thread about muscle culture and seahorse etc, I haven't gotten to read the paper yet but useful things to look into would be a) whether activity matched controls b) passaging/handling of the cultures (primary muscle cell phenotypes change with doublings - is that what has been used? havent looked in detail at what was cultured) c) absolute basal oxygen consumption rate values not being negative or close to 0 - if reported as normalised to cell number or stained DNA fluorescence or total protein this might be harder to glean d) not a huge amount of variability after ionophore injection (if used, ia assume so) this can cause cells to swell up and detach which confounds measurements, many aspects of the protocol have to be optimised to avoid it such as instrument mixing protocol, choice or presence of adherence matrix, incubation times, etc
sorry to offload homework, under the pump
my interactions with the lead author leave me confident that pitfalls would have been avoided but yes those are the first things that spring to mind when it comes to evaluating this kind of work. there are others but too technical to explain briefly
sorry to offload homework, under the pump
my interactions with the lead author leave me confident that pitfalls would have been avoided but yes those are the first things that spring to mind when it comes to evaluating this kind of work. there are others but too technical to explain briefly
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