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MCP-1 is Increased in Patients with CFS and FM, whilst several other immune markers are significantly lower than healthy controls, 2020, Groven et al

Discussion in 'BioMedical ME/CFS Research' started by Kalliope, Apr 2, 2020.

  1. Kalliope

    Kalliope Senior Member (Voting Rights)

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    Elsevier - Brain, Behaviour, & Immunity - Health
    MCP-1 is Increased in Patients with CFS and FM, whilst several other immune markers are significantly lower than healthy controls - Nina Groven, Egil Andreas Fors, Astrid Kamilla Stunes, Solveig Klæbo Reitan

    Abstract
    The role of the immune system in the pathogenesis of Fibromyalgia (FM) and Chronic fatigue syndrome (CFS) is not clear. We have previously reported increased levels of C-reactive protein (CRP) in these patient groups compared to healthy controls and wanted to further explore the levels of circulating immune markers in these populations.

    The population consisted of three groups, 58 patients with FM, 49 with CFS and 54 healthy controls. All participants were females aged 18–60. Patients were recruited from a specialised university hospital clinic and controls were recruited by advertisement among the staff and students at the hospital and university. Plasma levels of Interferon (IFN)-γ, Interleukin (IL)-1β, IL-1ra, IL-4, IL-6, IL-8, IL-10, IL-17, Interferon gamma-induced protein (IP)-10, Monocyte Chemoattractant Protein (MCP)-1, Transforming Growth Factor (TGF)-β1, TGF-β2, TGF-β3 and Tumour Necrosis Factor (TNF)-α were analysed by multiplex. Differences between the three groups CFS, FM and controls, were analysed by Kruskal Wallis tests.

    MCP-1 was significantly increased in both patient groups compared to healthy controls. IL-1β, Il-4, IL-6, TNF-α, TGF-β1, TGF-β2, TGF-β3, IL-10 and IL17 all were significantly lower in the patient groups than healthy controls. IFN-γ was significantly lower in the FM group. For IL-8, IL-10 and IL-1ra there were no significant difference when controlled for multiple testing.

    In conclusion, in our material MCP-1 seems to be increased in patients both with CFS and with FM, while several other immune markers are significantly lower in patients than controls.
     
  2. Kalliope

    Kalliope Senior Member (Voting Rights)

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    At least one of the co authors, Egil A. Fors, is a strong defender of the biopsychosomatic approach to ME.

    Nina Groven, Egil A. Fors and Solveig Klæbo Reitan published the study "Patients with Fibromyalgia and Chronic Fatigue Syndrome show increased hsCRP compared to healthy controls" last year (discussed here).
     
  3. Andy

    Andy Committee Member & Outreach

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    ACR is Fibromyalgia criteria.
    Shame that they didn't retrospectively run the participants through any other criteria.

    From the Limitations section (my bolding).
    Fukuda strict! :laugh:
     
  4. wigglethemouse

    wigglethemouse Senior Member (Voting Rights)

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    It always puzzles me why they don't show a scatter plot of the results to help with visualisation of the data. They claim a significance of P<0.001 but the standard deviation is pretty high, meaning there is a lot of overlap of the data points between the groups. A picture would give a better idea how significant the overlap was or not ..........

    I remember a talk given by Montoya where he said that for Cytokine analysis, patient and control samples had to be mixed within the same centrifuge and processed at the same time, to remove processing variations. He, and others I've listened to, have said the same equipment should be used for all samples in the study, to remove equipment variation. This paper does not go into that level of detail.

    Unfortunately Cytokine studies seem pretty unrepeatable by others as reported in Michael Van Elzakker's paper. So take with a pinch of salt.
     
    Michelle, Andy, Trish and 2 others like this.

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