Background The metabolic trap hypothesis being investigated by the OMF is currently investigating IDO2, with the Severely Ill Big Data Project finding IDO2 mutations in the 20 patients ill with M.E. This question is about how someone can check if they have the mutations found based on genetic data analyzed by popular saliva tests. Gene data Many people have had their genetic data analyzed from saliva tests, eg by 23andMe or Ancestry DNA. This results in a large file that can be searched for different SNPs. Various projects have collected this data eg Nova Southeastern's ME/CFS Gene Study run by Nancy Klimas. Question 1 I believe 5 or 6 IDO2 mutations were identified, that meant IDO2 was either not working at all or not working properly. How can you find a list of these to compare with you own genetic data? What seems to be needed is the rsid and the corresponding allele values. Source 1: The diagram on p5 may contain part of the answer, but what exactly to look for in the file? https://www.omf.ngo/wp-content/uploads/2018/11/Edited-Robert-Phair-Metabolic-Trap.pdf Source 2: Diagnostics 2019, 9(3), 82; https://doi.org/10.3390/diagnostics9030082 The IDO Metabolic Trap Hypothesis for the Etiology of ME/CFS Table 1. Common and rare mutations in IDO2 identified as damaging 3. eg for mutation R248W - snp rs10109853 Allele ref > alt C > T exon 9 Min pop AF 0.418 (minimum alternate allele frequency (expressed as a fraction) reported  for any sampled population) Max pop AF 0.487 (maximum alternate allele frequency reported for any sampled population) SIFT damaging PROVEAN deleterious POLYPHEN probably damaging so with your genetic data, suppose it says: rsid rs10109853 chromosome 1 allele 1 G allele 2 G * how does this compare to the damaging mutation? *Do alleles 1 and 2 need to be any letter between C and T? *Does the exon need to match the chromosome number to have that mutation? *Are the min / max values relevant, or is that missing from the genetic data analysis? *Where to look for the R248W? Is this the name of a gene or something else? Question 2 Are there any particular values that should be there, that would cause a problem in IDO2 if they are missing? Please forgive me for any basic misunderstandings or confusion and the many wrong words. I am extremely brain fogged and it's taken me so very long just to get this far.