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Cytokine signatures in chronic fatigue syndrome patients (2017) Roerink

Discussion in 'BioMedical ME/CFS Research' started by Cheesus, Dec 30, 2017.

  1. Cheesus

    Cheesus Established Member (Voting Rights)

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    Cytokine signatures in chronic fatigue syndrome patients: a Case Control Study and the effect of anakinra treatment

    Abstract
    Background
    Cytokine disturbances have been suggested to be associated with the Chronic Fatigue Syndrome/Myalgic encephalomyelitis (CFS/ME) for decades.

    Methods
    Fifty female CFS patients were included in a study on the effect of the interleukin-1-receptor antagonist anakinra or placebo during 4 weeks. EDTA plasma was collected from patients before and directly after treatment. At baseline, plasma samples were collected at the same time from 48 healthy, age-matched female neighborhood controls. A panel of 92 inflammatory markers was determined in parallel in 1 μL samples using a ‘proximity extension assay’ (PEA) based immunoassay. Since Transforming growth factor beta (TGF-β) and interleukin-1 receptor antagonist (IL-1Ra) were not included in this platform, these cytokines were measured with ELISA.

    Results
    In CFS/ME patients, the ‘normalized protein expression’ value of IL-12p40 and CSF-1 was significantly higher (p value 0.0042 and 0.049, respectively). Furthermore, using LASSO regression, a combination of 47 markers yielded a prediction model with a corrected AUC of 0.73. After correction for multiple testing, anakinra had no effect on circulating cytokines. TGF-β did not differ between patients and controls.

    Conclusions
    In conclusion, this study demonstrated increased IL-12p40 and CSF-1 concentrations in CFS/ME patients in addition to a set of predictive biomarkers. There was no effect of anakinra on circulating cytokines other than IL-1Ra.

    https://translational-medicine.biomedcentral.com/articles/10.1186/s12967-017-1371-9
     
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  2. Cheesus

    Cheesus Established Member (Voting Rights)

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    Interesting quote:

     
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  3. MeSci

    MeSci Senior Member (Voting Rights)

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    The authors are
    Megan E. Roerink, Hans Knoop, Ewald M. Bronkhorst, Henk A. Mouthaan, Luuk J. A. C. Hawinkels, Leo A. B. Joosten and Jos W. M. van der Meer

    Journal of Translational Medicine 2017 15:267

    https://doi.org/10.1186/s12967-017-1371-9

    © The Author(s) 2017

    Received: 20 October 2017

    Accepted: 18 December 2017

    Published: 29 December 2017

    Knoop has an interesting collection of articles, from the absurd to the credible.
     
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  4. Webdog

    Webdog Senior Member (Voting Rights)

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    IL-12 therapy is also used in non-Hodgkin T-cell Lymphoma, which my sibling died of in her mid 20s.

    https://www.researchgate.net/public...eukin-12_Therapy_of_Cutaneous_T_Cell_Lymphoma

    Also, IL-12 has been implicated in insulin-dependent Type 1 diabetes mellitus , which one of my parents suffered from.

    https://www.researchgate.net/publication/12154344_Interleukin_12_and_autoimmune_diabetes

    Coincidence?
     
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  5. Forbin

    Forbin Senior Member (Voting Rights)

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    In the 2015 Columbia paper, IL-12p40 showed very significant differences between controls and both long- and short-term patients (DN p=0.0012 and UP p= 0.0009. respectively) and between the two patient subsets themselves (p<0.0001).

    The finding was far less significant when short- and long-term patients were combined and measured against controls (DN p=0.0803).


    http://advances.sciencemag.org/content/1/1/e1400121
     
    Last edited: Dec 31, 2017
    pteropus, Inara and Esther12 like this.

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