Post moved from Mitochondrial dysfunction and the pathophysiology of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) 2012 Booth et al It was recently prompted by Joan's comment on cell-free DNA. Coupled with more recent papers on neutrophils and NETs in LC that were on my radar. Eg Plasma proteome of Long-COVID patients indicates HIF-mediated vasculo-proliferative disease with impact on brain and heart function (2023) — [Maybe we should move these posts to the Neutrophil Extracellular Traps thread or a more general cell-free DNA thread?] Same idea and could be straightforward. I note JE has commented on problems with neutrophils and NETs evaluation and theory.
A relevant thread: Mitochondrial dysfunction and the pathophysiology of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) 2012 Booth et al This refers to the Myhill-promoted Test that included cell-free DNA levels. And another: Elevated cfDNA after exercise is derived primarily from mature neutrophils, with a minor contribution of cardiomyocytes, 2023, Fridlich et al This study in health people found massive increases in cell-free DNA following exercise, mostly derived from neutrophils. The cell-free DNA has a very short life, with levels returning to normal with an hour of exercise. It uses a technique to identify the cells that the cell-free DNA came from, which could be very interesting to apply to ME/CFS samples.
Potentially an interesting area for study, along with other known responses to exercise and recovery. It's been a while back that I read up about free actin and myosin levels in the blood plasma. From memory, there was one small study completed in Spain about this in pwME. I contacted the author at the time it was published and they didn't reply. I discussed this with Willy Weir too. I don't recall seeing anymore about this since. It would be illuminating to test pwME pre and post exercise challenge on these well-known and straightforward to test methods. I suspect these would show up that all is not as it should be.
So it would be important to follow this at eg 1, 4, 24, 48 hours. The short half life in the normal situation will be (partly?) predicated on the amount of DNases, which is increased with regular training - clearly not something we can do once the disease is established, with limiting PEM.
Epigenetic liquid biopsies reveal elevated vascular endothelial cell turnover and erythropoiesis in asymptomatic COVID-19 patients (2023, Preprint: BioRxiv)
I can't judge the relevance of this work, especially not without reading it, but just reading the abstract it seems to be slightly in line with Rob Wüsts work?
