An investigation into the modulation of T cell phenotypes by amitriptyline and nortriptyline, 2019, Conroy et al

Dolphin · Dec 28, 2019

Possibly of interest, given how many patients can be on these types of drugs. Though I don't really understand what the implications might be myself without reading the full paper.

https://www.sciencedirect.com/science/article/abs/pii/S0924977X1931870X

European Neuropsychopharmacology
Available online 24 December 2019
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An investigation into the modulation of T cell phenotypes by amitriptyline and nortriptyline
Author links open overlay panelJonathanRoydsa
Melissa J.ConroybMargaret R.DunnebConnailMcCroryaJoanneLysaghtb
https://doi.org/10.1016/j.euroneuro.2019.12.106 Get rights and content

Abstract
Amitriptyline is prescribed for treating the symptoms of neuroinflammatory disorders including neuropathic pain and fibromyalgia. As amitriptyline has evidence of modulating the neuroimmune interface; the effects of amitriptyline treatment on T-cell phenotype and function were examined in vitro. Peripheral blood mononuclear cells(PBMCs) were isolated and treated with amitriptyline, nortriptyline and a combination of both drugs. Toxicity for T-cells was assessed by Annexin V/Propidium Iodide staining. Activation status and cytokine expression by T-cells post treatment was assessed by flow cytometry. The levels of secreted cytokines, chemokines and neurotrophins were measured by ELISA in the supernatants. There was no significant increase in T-cell death following 24 or 48 h compared to controls. There were significantly lower frequencies of CD8+ T-cells after treatment with amitriptyline, nortriptyline and a combination of both compared to a Vehicle Control(VC)(p<0.001). The frequencies of naive CD8+CD45RA+ cells were significantly lower after amitriptyline, nortriptyline and a combination of both (p<0001). The frequencies of CD27+CD4+(p<0.05) and CD27+CD8+(p<0.01) T-cells were also significantly lower following combination drug treatment. Significantly lower frequencies of IFN-γ-producing CD8+ T-cells were observed with all treatment combinations(p<0.05) and frequencies of IL-17-producing CD4+ and CD8+ T-cells were significantly lower following amitriptyline treatment (p<0.05). Frequencies of Natural Killer T-cells were significantly higher following treatment with nortriptyline (p<0.05). Significantly higher levels of IL-16 (p<0.001) and lower levels of TNF-β (p<0.05) were observed in supernatants. This data indicates that both amitriptyline and nortriptyline modulate the phenotype and function of T-cells and this may have clinical relevance in the pathologies of its off-label applications.

Keywords
Amitriptyline
Nortriptyline
T cells
Neuropathic pain
Neuroimmune interface
Neuroinflammation

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Amw66 · Dec 28, 2019

How does this tie in with previous T cell research?

Do we know if other T cell research involved anyone taking Ami / Notriptyline which may have affected results given above?

Jaybee00 · Dec 28, 2019

Interesting. I remember discussion about amitrip. on the Howard Bloom thread here—the MECFS guy that does 700 pushups.

Lisa108 · Dec 29, 2019

Sci-hub link here

If this link doesn't work in your country, try to insert the doi-number (in this case: 10.1016/j.euroneuro.2019.12.106) here.

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