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Systematic analysis of the cerebrospinal fluid proteome of fibromyalgia patients

Discussion in ''Conditions related to ME/CFS' news and research' started by Milo, May 6, 2018.

  1. Milo

    Milo Senior Member (Voting Rights)

    Fibromyalgia (FM) is a syndrome characterized by widespread muscular pain, fatigue and functional symptoms, which is known to be difficult to diagnose as the various symptoms overlap with many other conditions.

    Currently, there are no biomarkers for FM, and the diagnosis is made subjectively by the clinicians.

    We have performed shotgun proteomics on cerebrospinal fluid (CSF) from FM patients and non-pain controls to find potential biomarker candidates for this syndrome.

    Based on our multivariate and univariate analyses, we found that the relative differences in the CSF proteomebetween FM patients and controls were moderate.

    Four proteins, important to discriminate FM patients from non-pain controls, were found: Apolipoprotein C-III, Galectin-3-binding protein, Malate dehydrogenase cytoplasmic and the neuropeptide precursor protein ProSAAS.

    These proteins are involved in lipoprotein lipase (LPL) activity, inflammatory signaling, energy metabolism and neuropeptide signaling.

    Fibromyalgia is present in as much as 2% of the population, causing pain, stiffness, and tenderness of the muscles.

    Upon accurate diagnostic, nonpharmacological and pharmacological therapies can be used to alleviate pain and manage other symptoms.

    However, lack of objective, universal applicable diagnostic criteria as well as vague and diffused symptoms, have made diagnosis difficult.

    In this context, our findings can shed light on potential value of CSF proteome for objectively diagnosing FM.


    Proteomics analysis of the cerebrospinal fluid of patients suffering from fibromyalgia compared to non-pain controls

    Stringent statistical approach using multivariate and univariate methods

    Alteration of apolipoprotein C-III, galectin-3-binding protein, malate dehydrogenase cytoplasmic and ProSAAS in fibromyalgia

    Demonstrated potential disturbance in neuroinflammation, neuroendocrine and energy metabolism in fibromyalgia patients

    Link to paper (paywall) https://www.sciencedirect.com/science/article/pii/S1874391918301738
  2. Trish

    Trish Moderator Staff Member

    It's hard to tell from only reading the abstract, but this looks interesting. I hope someone can get funding to see whether they can replicate these findings, and test for the same things in ME.

    They found 4 proteins that were at different levels in 39 FM patients compared with 38 non pain controls, but they don't say in the abstract how many proteins they tested or what significance level was reached, and how much overlap there was between healthy and FM measurements - was this just a case of p-hacking - searching a huge set of data for the few that dropped below the 5% significance level by chance - or do these results mean something?
  3. Milo

    Milo Senior Member (Voting Rights)

    I hope so too. However this involves an invasive procedure, the blurrying of the fibromyalgia case definition and the lack of medical home are all barriers to a replication study.

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