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Single-cell RNA-Seq reveals intracellular microbial diversity within immune cells during SARS-CoV-2 infection and recovery, 2023, Sunita Yadav et al

Discussion in 'Epidemics (including Covid-19, not Long Covid)' started by Mij, Dec 25, 2023.

  1. Mij

    Mij Senior Member (Voting Rights)

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    Highlights
    • 76 bacterial species of 15 genera across healthy, COVID-19-positive, and recovered individuals
    • Higher abundance of opportunistic intracellular bacteria in recovered group
    • Higher microbial abundance within antigen-presenting cells of COVID-19 patients
    • Differential microbial abundance in Treg, memory T, and platelets in COVID-19
    Summary
    Intracellular microorganisms, like viruses, bacteria, and fungi, pose challenges in detection due to their non-culturable forms. Transcriptomic analysis at cellular level enables exploration of distributions and the impact of these microorganisms on host cells, a domain that remains underexplored because of methodological limitations.

    Single-cell technology shows promise in addressing this by capturing polyadenine-tailed transcripts, because recent studies confirmed polyadenylation in microbial transcriptomes. We utilized single-cell RNA-seq from PBMCs to probe intracellular microbes in healthy, SARS-CoV-2-positive, and recovered individuals.

    Among 76 bacterial species detected, 16 showed significant abundance differences. Buchnera aphidicola, Streptomyces clavuligerus, and Ehrlichia canis emerged significantly in memory-B, Naïve-T, and Treg cells. Staphylococcus aureus, Mycoplasma mycoides, Leptospira interrogans, and others displayed elevated levels in SARS-CoV-2-positive patients, suggesting possible disease association.

    This highlights the strength of single-cell technology in revealing potential microorganism’s cell-specific functions. Further research is essential for functional understanding of their cell-specific abundance across physiological states.

    https://www.cell.com/iscience/fullt...m/retrieve/pii/S2589004223024343?showall=true
     
  2. SNT Gatchaman

    SNT Gatchaman Senior Member (Voting Rights)

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    Introduction

     
    Sean likes this.
  3. SNT Gatchaman

    SNT Gatchaman Senior Member (Voting Rights)

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    Differential presence of non-human reads in healthy, SARS-CoV-2-positive, and recovered individuals

     
    Sean and LarsSG like this.
  4. SNT Gatchaman

    SNT Gatchaman Senior Member (Voting Rights)

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    Differential abundance of bacterial species in COVID-19-infected and recovered individuals versus healthy individuals

     
    Sean and LarsSG like this.
  5. SNT Gatchaman

    SNT Gatchaman Senior Member (Voting Rights)

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    Immune-cell-type-specific significant bacteria and their potential roles

     
  6. SNT Gatchaman

    SNT Gatchaman Senior Member (Voting Rights)

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    Discussion

     
    rvallee likes this.
  7. SNT Gatchaman

    SNT Gatchaman Senior Member (Voting Rights)

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    This study needs to be replicated in healthy vs recovered/asymptomatic vs LC ± vs acute.
     
    rvallee, Mij, obeat and 1 other person like this.

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