Release of extracellular vesicle miR-494-3p by ARPE-19 cells with impaired mitochondria, 2020, Ahn et al

Andy · Apr 3, 2020

Only looking at the mitochondria of cells in the eye but thought it was potentially interesting none the less.

Highlights
• miR-494-3p is enriched in the mitochondria of ARPE-19 cells.
• miR-494-3p regulates mitochondrial function in ARPE-19 cells.
• EV miR-494-3p released by ARPE-19 cells indicates mitochondrial dysfunction.

Abstract
Background
Mitochondrial function in retinal pigmented epithelial (RPE) cells and extracellular vesicle (EV) formation/release are related through the lysosomal and exocytotic pathways that process and eliminate intracellular material, including mitochondrial fragments. We propose that RPE cells with impaired mitochondria will release EVs containing mitochondrial miRNAs that reflect the diminished capacity of mitochondria within these cells.

Methods
We screened ARPE-19 cells for miRNAs that localize to the mitochondria, exhibit biological activity, and are present in EVs released by both untreated cells and cells treated with rotenone to induce mitochondrial injury. EVs were characterized by vesicle size, size distribution, presence of EV biomarkers: CD81, CD63, and syntenin-1, miRNA cargo, and number concentration of EVs released per cell.

Results
We found that miR-494-3p was enriched in ARPE-19 mitochondria. Knockdown of miR-494-3p in ARPE-19 cells decreased ATP production and mitochondrial membrane potential in a dose-dependent manner, and decreased basal oxygen consumption rate and maximal respiratory capacity. Increased number concentrations of EVs released per cell and elevated levels of miR-494-3p in EVs released from ARPE-19 cells treated with rotenone were also measured.

Conclusions
ARPE-19 mitochondrial function is regulated by miR-494-3p. Elevated levels of miR-494-3p in EVs released by ARPE-19 cells indicate diminished capacity of the mitochondria within these cells.

General significance
EV miR-494-3p is a potential biomarker for RPE mitochondrial dysfunction, which plays a central role in non-neovascular age-related macular degeneration, and may be a diagnostic biomarker for monitoring the spread of degeneration to neighboring RPE cells in the retina.
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Paywall, https://www.sciencedirect.com/science/article/abs/pii/S0304416520301100
Not available via Sci hub at time of posting.

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Release of extracellular vesicle miR-494-3p by ARPE-19 cells with impaired mitochondria, 2020, Ahn et al

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Release of extracellular vesicle miR-494-3p by ARPE-19 cells with impaired mitochondria, 2020, Ahn et al

Andy Committee Member

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