Preprint Most axonal mitochondria in cortical pyramidal neurons lack mitochondrial DNA and consume ATP 2024 Hirabayashi et al

Abstract

In neurons of the mammalian central nervous system (CNS), axonal mitochondria are thought to be indispensable for supplying ATP during energy-consuming processes such as neurotransmitter release. Here, we demonstrate using multiple, independent, in vitro and in vivo approaches that the majority (~80-90%) of axonal mitochondria in cortical pyramidal neurons (CPNs), lack mitochondrial DNA (mtDNA). Using dynamic, optical imaging analysis of genetically encoded sensors for mitochondrial matrix ATP and pH, we demonstrate that in axons of CPNs, but not in their dendrites, mitochondrial complex V (ATP synthase) functions in a reverse way, consuming ATP and protruding H+ out of the matrix to maintain mitochondrial membrane potential. Our results demonstrate that in mammalian CPNs, axonal mitochondria do not play a major role in ATP supply, despite playing other functions critical to regulating neurotransmission such as Ca2+ buffering.

https://www.biorxiv.org/content/10.1101/2024.02.12.579972v1

 

Valuable discovery. That should reduce the number of overly simplistic theories involving neurons and mitochondria.

 

Complex V.
Interesting if there was overcompensation in complex 1 as has been found on ME research.