Metabolic profiling indicates impaired pyruvate dehydrogenase function in ME/CFS, 2016, Fluge, Mella et al

adreno · Oct 31, 2017

Another important one:

Øystein Fluge, Olav Mella, et al.

Abstract

Myalgic encephalopathy/chronic fatigue syndrome (ME/CFS) is a debilitating disease of unknown etiology, with hallmark symptoms including postexertional malaise and poor recovery. Metabolic dysfunction is a plausible contributing factor. We hypothesized that changes in serum amino acids may disclose specific defects in energy metabolism in ME/CFS. Analysis in 200 ME/CFS patients and 102 healthy individuals showed a specific reduction of amino acids that fuel oxidative metabolism via the TCA cycle, mainly in female ME/CFS patients. Serum 3-methylhistidine, a marker of endogenous protein catabolism, was significantly increased in male patients. The amino acid pattern suggested functional impairment of pyruvate dehydrogenase (PDH), supported by increased mRNA expression of the inhibitory PDH kinases 1, 2, and 4; sirtuin 4; and PPARδ in peripheral blood mononuclear cells from both sexes. Myoblasts grown in presence of serum from patients with severe ME/CFS showed metabolic adaptations, including increased mitochondrial respiration and excessive lactate secretion. The amino acid changes could not be explained by symptom severity, disease duration, age, BMI, or physical activity level among patients. These findings are in agreement with the clinical disease presentation of ME/CFS, with inadequate ATP generation by oxidative phosphorylation and excessive lactate generation upon exertion.
Click to expand...

https://insight.jci.org/articles/view/89376

Log in or Sign up

Metabolic profiling indicates impaired pyruvate dehydrogenase function in ME/CFS, 2016, Fluge, Mella et al

adreno Senior Member (Voting Rights)

Share This Page

Log in or Sign up

Metabolic profiling indicates impaired pyruvate dehydrogenase function in ME/CFS, 2016, Fluge, Mella et al

adreno Senior Member (Voting Rights)

Share This Page

Useful Searches