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Broadband electrical impedance as a novel characterization of oxidative stress in single L6 muscle cells, 2021, Ferguson et al

Discussion in 'BioMedical ME/CFS Research' started by Dolphin, May 29, 2021.

  1. Dolphin

    Dolphin Senior Member (Voting Rights)

    Messages:
    3,155
    From: Dr. Marc-Alexander Fluks

    Source: Analytica Chimica Acta
    Preprint
    Date: May 25, 2021
    URL:
    https://www.sciencedirect.com/science/article/abs/pii/S0003267021005043


    Broadband electrical impedance as a novel characterization of
    oxidative stress in single L6 skeletal muscle cells
    -------------------------------------------------------------
    Caroline Ferguson(a), Niccolo Pini(b), Xiaotian Du(c), Marco Farina(d), James M.C.H wang(c), Tiziana Pietrangelo(b), Xuanhong Chenga(e,*)
    a Department of Bioengineering, Lehigh University, Bethlehem, PA, USA
    b Department of Neuroscience, Imaging, and Clinical Science, University G. D'Annuzio, Chieti-Pescara, Italy
    c Department of Electrical Engineering, Lehigh University, Bethlehem, PA, USA
    d Department of Engineering of Information, University Politecnica, Delle Marche, Marche, Italy
    e Department of Materials Science and Engineering, Lehigh University, Bethlehem, PA, USA
    * Corresponding author. Department of Bioengineering, Lehigh University, Bethlehem, PA, USA. Email: xuc207@lehigh.edu

    Received 4 May 2021
    Revised 18 May 2021
    Accepted 20 May 2021
    Available online 25 May 2021.


    Abstract

    Oxidative stress (OS) is one of the leading causes of cytotoxicity and is linked to many human physio-pathological conditions. In particular, myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) induced by OS is debilitating to quality of life, while no clear biological markers have been identified for diagnostic measures. Recently, impedance measurements of peripheral blood cells of ME/CFS patients have been shown as a promising approach to diagnose the disease.

    Inspired by this study and aiming to interrogate muscle cells directly, we investigated if broadband measurements of single muscle cells could differentiate normal and oxidatively stressed cell populations. We first optimized a protocol through H2O2 treatment to introduce oxidative stress to cultured rat L6 skeletal muscle cells. The treated cells were further characterized through broadband impedance spectroscopy of single cells using a microfluidic lab-on-a-chip system. The resulting dielectric properties of cytoplasm permittivity and conductivity are electrically distinct from normally cultured cells. The reflection and transmission coefficients, ΔS11 and ΔS21, of the normal cells are tightly clustered and closely resemble those of the cell-free solution across the frequency range of 9 kHz to 9 GHz. On the other hand, dielectric properties of the oxidized cells have a wide distribution in the GHz range, deviating both in the positive and negative directions from the normally cultured cells.

    Simulation results guide our hypothesis that the dielectric differences could be linked to ion alterations, while calcium imaging directly supports the contribution of calcium flux to the observed deviation of S parameters. The unique electrical profile associated with oxidized cells in the GHz frequencies provide a framework for future development of technologies to diagnose oxidative-stress related diseases such as ME/CFS.

    Keywords: Oxidative stress, broadband electrical sensing, dielectric,
    calcium signaling, microfluidic, single cell

    --------
    (c) 2021 Elsevier B.V.
     
    Last edited by a moderator: May 29, 2021
    sebaaa, Barry, Wyva and 4 others like this.
  2. alex3619

    alex3619 Senior Member (Voting Rights)

    Messages:
    1,721
    Issues with impedance, alone, are not what is being measured in ME. Impedence on salt stressed cells is being measured. This may be an important difference. Characterizing why the problem occurs is important though, and oxidative stress might play some role, or not.
     
    Last edited: May 30, 2021
    sebaaa, Michelle, alktipping and 2 others like this.
  3. Hutan

    Hutan Moderator Staff Member

    Messages:
    17,430
    Location:
    New Zealand
    It's nice though to see the development of an approach to differentiate a normal cell from a cell that has suffered oxidative stress.

    I guess the next step for this team will be to use their approach to look at cells from people with ME/CFS to see if they look more like the healthy cells or the cells treated with H2O2.
     
    sebaaa, Sean, Michelle and 4 others like this.

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