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Altered endothelial dysfunction-related miRs in plasma from ME/CFS patients, 2021, Blauensteiner et al

Discussion in 'BioMedical ME/CFS News' started by InitialConditions, May 19, 2021.

  1. InitialConditions

    InitialConditions Senior Member (Voting Rights)

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    https://www.nature.com/articles/s41598-021-89834-9

    ABSTRACT
    Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a complex disease characterized by unexplained debilitating fatigue. Although the etiology is unknown, evidence supports immunological abnormalities, such as persistent inflammation and immune-cell activation, in a subset of patients. Since the interplay between inflammation and vascular alterations is well-established in other diseases, endothelial dysfunction has emerged as another player in ME/CFS pathogenesis. Endothelial nitric oxide synthase (eNOS) generates nitric oxide (NO) that maintains endothelial homeostasis. eNOS is activated by silent information regulator 1 (Sirt1), an anti-inflammatory protein. Despite its relevance, no study has addressed the Sirt1/eNOS axis in ME/CFS. The interest in circulating microRNAs (miRs) as potential biomarkers in ME/CFS has increased in recent years. Accordingly, we analyze a set of miRs reported to modulate the Sirt1/eNOS axis using plasma from ME/CFS patients.

    Our results show that miR-21, miR-34a, miR-92a, miR-126, and miR-200c are jointly increased in ME/CFS patients compared to healthy controls. A similar finding was obtained when analyzing public miR data on peripheral blood mononuclear cells. Bioinformatics analysis shows that endothelial function-related signaling pathways are associated with these miRs, including oxidative stress and oxygen regulation. Interestingly, histone deacetylase 1, a protein responsible for epigenetic regulations, represented the most relevant node within the network. In conclusion, our study provides a basis to find endothelial dysfunction-related biomarkers and explore novel targets in ME/CFS.
     
    ahimsa, Milo, DokaGirl and 30 others like this.
  2. InitialConditions

    InitialConditions Senior Member (Voting Rights)

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    Discussion from author here:


    Lots of work/findings on endothelial cells / function recently, including in POTS. I have no idea what they are / do. :-$
     
    Milo, merylg, DokaGirl and 23 others like this.
  3. Andy

    Andy Committee Member (& Outreach when energy allows)

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    From the paper, for info.

    "Acknowledgements

    This study was supported by ME Research UK (SCI charity number SC036942) and carried out using samples from the UK ME/CFS Biobank, which were processed and stored at the UCL-RFH BioBank). The authors thank all the study participants for donating their blood to the UK ME/CFS Biobank (UKMEB) and also the ME/CFS community who raised funds to facilitate the UKMEB projects. The authors also want to thank the ME/CFS Society Austria (Österreichische Gesellschaft für ME/CFS, CFS-Hilfe), and the FH JOANNEUM University of Applied Sciences for supporting gran applications. FW thanks Mr. Mika Remes (QIAGEN) for his technical assistance. Finally, FW especially thanks Mr. Luis Westermeier for his support and encouragement throughout this study."
     
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  4. Lindberg

    Lindberg Established Member

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    Could this have anything to do with anti-ETAR antibodies? Or would that be totally irrelevant for this matter?
     
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  5. Hutan

    Hutan Moderator Staff Member

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    That a nice acknowledgements section. It's good to see researchers from Austria and Chile working on ME/CFS biology.

    J. Blauensteiner

    Institute of Biomedical Science, Department of Health Studies, FH Joanneum University of Applied Sciences, Graz, Austria

    R. Bertinat

    Centro de Microscopía Avanzada, CMA-BIO BIO, Facultad de Ciencias Biológica, Universidad de Concepción, Concepción, Chile

    L. E. León

    Instituto de Ciencias Biomédicas, Facultad de Ciencias de La Salud, Universidad Autónoma de Chile, Santiago, Chile

    M. Riederer

    Institute of Biomedical Science, Department of Health Studies, FH Joanneum University of Applied Sciences, Graz, Austria

    N. Sepúlveda

    Department of Infection Biology, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK
    Centro de Estatística e Aplicações, Universidade de Lisboa, Lisbon, Portugal
    Institute of Medical Immunology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität (FU) Berlin, Humboldt-Universität Zu Berlin and Berlin Institute of Health (BIH), Berlin, Germany

    F. Westermeier

    Institute of Biomedical Science, Department of Health Studies, FH Joanneum University of Applied Sciences, Graz, Austria
    Centro Integrativo de Biología y Química Aplicada (CIBQA), Universidad Bernardo O´Higgins, Santiago, Chile
     
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  6. Hutan

    Hutan Moderator Staff Member

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    This study - miRNA in plasma
    Screen Shot 2021-05-20 at 6.45.49 AM.png


    Analysis of previously published data of miRNA in PBMCs:
    Screen Shot 2021-05-20 at 6.52.03 AM.png

    There's overlap between levels in pwME and healthy controls of course, but average levels do seem to be higher and this is consistent in another study. I haven't read the paper yet, but it looks interesting.
     
  7. Grigor

    Grigor Senior Member (Voting Rights)

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  8. dreampop

    dreampop Senior Member (Voting Rights)

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    Histone deacetylase has come up a few times over the past decade.

    1) In 2011, Lenny Jason found, in this paper

    2) A group at Brussels has set up a study that that will look at HDAC genes under PEM.

    https://clinicaltrials.gov/ct2/show/NCT04378634

    3) One of the more interesing papers, imo, of recent years was Neil McGregor's "Hypermetabolism" findings, which included blood and urine testing and indicated, amongst other things, problems with purine metabolism.

     
    Milo, Michelle, Grigor and 4 others like this.
  9. dreampop

    dreampop Senior Member (Voting Rights)

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    I believe there might be use in going to see if it pinged any other epigentic me/cfs studies, but I'm too tired to do that atm.
     
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