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They use 200mM NaCl in the nanoneedle paper which is why I mentioned that number above. You would think that would be enough to really hurt, as I say it seems to affect cell survival in one study for a T cell line but another cancer cell line HeLa cells don't mind - but then these are very hardy...

Might depend on the cell type but I think cells have salt concentrations of about 150mM and so like to sit in an isotonic buffer of about 150mM NaCl. I've tried this before when you put suspended fibroblast cells in 300mM NaCl - a hypertonic solution - and they shrivel up dramatically as the...

I think A3

putting up posters, another old friend also saw my tweet and asked if they could help

Right, looking again at the diagram it seems both electrode terminals are located in a single projection that pokes into a channel roughly the width of a cell. There's probably 1 or 2 microns between the anode and cathode. I wish they could have shown a picture of the cells sitting in their...

My background is in molecular and computational biology, and not in electrical engineering but I did look into the nanoneedle a while back. The assay is super weird and not like something I've seen used in the field before. I suppose it most closely resembles a coulter counter, where you pass...

Setting up a biobank is important for sure but just logistically collecting serum is harder than collecting spit. It requires a phlebotomist to travel around collecting blood, and the blood needs processed the same day which requires basic lab things and a centrifuge to spin down the clot, and...

Thanks @Arvo that's interesting. As you say as they haven't published it they could have done anything, and 12 days wouldn't be long enough anyway. Asides from this paper I do think it's possible there are different types of patient that you could describe in terms of stability or frequency of...

From the authors response re actimetry, it sounds like they might have actually collected really valuable natural activity histories for n=114 patients for possibly 6 months (inferring from the methods in the abstract). They even give us a tantalizing snippet of differences they claim to observe...

Some commentary here

Genome-wide Association Study of Long COVID, Lammi, Ollila et al 2023 Abstract Infections can lead to persistent or long-term symptoms and diseases such as shingles after varicella zoster, cancers after human papillomavirus, or rheumatic fever after streptococcal infections(1,2). Similarly...

That's a good thought! It does seem related and it's cool they're doing this kind of research, sounds like they're going about it in an objective way. Will be cool to see what methods they use.

Adding blink counting functionality to IR glasses Supposedly when you are concentrating you blink less frequently, so I've added some extra code to the IR saccade counter set up above that tries to count blinking specifically. Blinks have a different kinetic profile to saccades as shown here...

Great commentary @Hutan . I think the most compelling work in the paper is on dUTPase effect on cytoskeleton and mitochondrial morphology - it's just that it has nothing to do with ME. It should probably be in a separate paper that whole section feels tenuously connected to everything else...

Not much more to say I think that hasn't been discussed already, but we now have the full data so can finally see if what we've heard him claim about differences in natural IgM levels stacks up. This I think is the key figure, where he has measured immunoglobulin levels with ELISA in a decently...

The figure to compare it to in the Goebel & Andersson paper would be figure 3 panels C and D: These show mechanical sensitivity and heat sensitivity as in the corresponding figure @me-cfs-skeptic just posted - but it is using a cold plate rather than a hot plate. The data here correspond to day...

This n=4 value is referring to the number of mice and not the number of patients so they are effectively technical replicates. Looking through the paper it's weirdly difficult to find out how many patients and controls they're using it's not even mentioned in the methods. They mention it only in...

@Andy or whoever may know - do you have a sense of how long recruitment is expected to continue for? I've persuaded my family to put up posters around pharmacies, GP, libraries where I live but they're being slow about it - is there much time?

Too long, didn't read: I've jerry-rigged some sensors to a pair of glasses and am able to count eye movements with them. It's certainly not perfect but it works pretty decently for how precarious this prototype set up appears. If you want to get a sense of its performance just look at the last...

Myself included I've had ME for something like 13 years and it took me a long time before I found a community (i.e this one) which is actually speaking soberly about ME - it's extremely refreshing. Exactly, what I'm really asking is whether there's a single person who actually fits the...