Using Single-Cell Raman Microspectroscopy to Profile Human Peripheral Blood Mononuclear Cells, 2025, Gan et al

Using Single-Cell Raman Microspectroscopy to Profile Human Peripheral Blood Mononuclear Cells

Elizabeth Gan, Megan Stoker, Edie Guo, Karl J. Morten & Jiabao Xu

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Abstract
A reliable, validated test would enhance our ability to treat and research chronic conditions. Early and accurate diagnosis would provide an entry point into clinical care, give access to benefits, remove the stigma associated with these conditions, and importantly, provide researchers with a fundamental tool they require to study these heterogeneous disorders.

In this chapter, we describe how Raman microspectroscopy can be utilised to study the biology of peripheral blood mononuclear cells (PBMCs) isolated from human blood samples. Using machine learning approaches, the data generated can be used to attempt to separate different patient and control groups, subgroups within a patient cohort, and identify differences in intracellular metabolites which may provide clues about disease mechanisms.

Link (SpringerLink) [Paywall]

 

Interesting that there’s a new paper on this. I look forward to finding out what it says and if they replicated previous findings. Does anyone have access?

Thread on the previous paper on this topic by this group
Developing a blood cell-based diagnostic test for ME/CFS using peripheral blood mononuclear cells, 2023, Xu, Morten et al

edit: oh I’ve just realised it’s a chapter from a book mentioned in his thread on research protocols
ME/CFS Springer Protocols