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Project : Droplet Digital PCR For Evaluation Of Associations Between Human Herpesvirus Infection And ME/CFS, Jackie Cliff 2020

Discussion in 'BioMedical ME/CFS Research' started by wigglethemouse, Jul 26, 2020.

  1. wigglethemouse

    wigglethemouse Senior Member (Voting Rights)

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    This study received significant funding from the US NIH but it doesn't seem a paper will be published. I'm guessing they only received funding for R21 to do feasibility work and that work allowed them to develop a test but only test a small cohort.

    US NIH Project Description
    Source : https://projectreporter.nih.gov/pro...dvalue=&ddsub=&cr=1&csb=default&cs=ASC&pball=
    US NIH Project Funding
    https://projectreporter.nih.gov/project_info_history.cfm?aid=9326134&icde=50995847
    2016 $140,688
    2017 $132,224

    There are no papers listed in the Results section. Instead it seems results are listed in the Outcomes section. Rather than 500 participants as quoted above it seems only 46 people participated and they say a large scale study is needed next.

    NIH Project Outcomes Listing
    https://projectreporter.nih.gov/project_info_outcomes.cfm?aid=9326134&icde=50995847
    The work was presented at the CMRC 2020 conference. I've not been able to watch yet (It is difficult for me to watch videos). It has been viewed only 135 times on YouTube so hopefully this post spreads more awareness.

    The UK ME/CFS Biobank: virological and immunological participant characterisation from Dr Jackie Cliff, London School of Hygiene & Tropical Medicine
    Code:
    https://youtu.be/52j0ivCIlQg
     
    Last edited: Jul 26, 2020
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  2. wigglethemouse

    wigglethemouse Senior Member (Voting Rights)

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    YouTube auto-generated transcript attached. I guess "I'm bar virus" is Epstein Barr Virus. Tee Hee. :laugh:
     

    Attached Files:

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  3. Hutan

    Hutan Moderator Staff Member

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    Thanks for highlighting this @wigglethemouse.

    I've had a quick listen. It didn't sound as though the team have found easily identified differences in herpes viral loads yet, but they have made a lot of progress in making studies like this feasible (with samples of urine and saliva posted; samples taken on good/bad days and aligned with symptom surveys; ability to extract pathogen DNA from the posted samples using droplet digital PCR which allows for good estimation of viral loads).

    There are hints of interesting findings including about MAIT cells, but the samples at the time of the presentation were too small for conclusions to be made.

    My conclusion was that this team sound as though they know what they are doing and they probably won't claim findings that aren't well based. So, I hope they get more funding and I look forward to hearing more from them.
     
    Last edited: Jul 26, 2020
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