Some people may have noticed that SNTG is systematically providing us with chapters from a Springer Protocol monograph on ME/CFS. https://link.springer.com/book/10.1007/978-1-0716-4498-0?page=1#toc This is a whole book on research methods for ME/CFS. Quite impressive. Who said nothing was happening in ME/CFS research ? _______________ Edit: List of threads on individual chapters (copied from post #13) Thread listing in no particular order — Profiling Antibody Reactivity to Gut Microbes in ME/CFS Patients Deep Immunophenotyping in ME/CFS Using Spectral Flow Cytometry Review of Neuroimaging Methods in ME/CFS Using the Ratio of Phosphorylated to Non-phosphorylated Forms of Stress Kinase PKR as a Potential Diagnostic Test for ME/CFS Quantitative Proteomics on Immune Cells of ME/CFS Patients Using SWATH-MS MicroRNA Profiling of Blood Extracellular Vesicles in ME/CFS Measurement of Genetic Variations in ME/CFS Patients in the IDO2 Gene Encoding an Enzyme Metabolizing Tryptophan Analysis of Transient Receptor Potential Ion Channels in ME/CFS Stress-Induced Changes in Immune Signatures in ME/CFS Patients Determined by Transcriptome Analysis Repeated Cardiopulmonary Exercise Testing of ME/CFS Patients Measuring Biomarkers of Oxidative Stress in ME/CFS Patients Mitochondrial Measures in Primary Cells Isolated from Patients with ME/CFS Application of DNA Methylome Analysis to Patients with ME/CFS Using Single-Cell Raman Microspectroscopy to Profile Human Peripheral Blood Mononuclear Cells Real-Time Measurement of Mitochondrial Function and Glycolysis in Lymphoblastoid Cell Lines
Does S4ME have funds to purchase a digital version of the book? Could each chapter then be shared individually for review on the forum, perhaps as members only posts if required to comply with publisher's requirements?
The editors (Warren Tate and Katie Peppercorn) did a monstrous amount of work for this and are to be congratulated. This has been in the works for a loooong time!
I agree re congratulations being in order. This looks to be a significant contribution, constituting a who's who of ME/CFS research.
Forgive me for my ignorance, can someone explain the significance of this? It looks to be a book describing methods of (largely already published) papers, is that correct? In which case what does this add? Is it more detail on those methods and how to reproduce them than is provided in the papers?
This sort of volume often appears at a stage when a biomedical problem has 'come of age' in the sense of having a community of people with different approaches all directed towards a common question - what is X? The value lies in the bringing together of all these approaches, more than just methods, so that new researchers and clinicians coming in to the field can see the resources available and how people have tried to apply them. It probably provides no new answers but sets out how everyone views the question. There was a volume like this by Leon Sokoloff for RA produced in about 1978. As a doctoral student it was my bible. It introduced me to work I would otherwise have had no inkling of.
Great, thanks for the context @Jonathan Edwards that’s very useful. Most fields have their bibles, I hope this can become that for this field. Perhaps with an addition or two over the coming years!
Looks interesting, good to see a focus on methodology. Most of these experiments, however, have not been able to detect clear signals of ME/CFS pathology or have not replicated by others. So perhaps new ME/CFS researchers should mostly try to use new and other methods rather than picking up these?
You could look at it like that, but then science always works by seeing how people have got with one method and from that deducing what might be a better one.
It does look interesting. Particularly interested to see that the Otago group has been working on epigenetics & DNA methylation analysis (given the Zhang results showed a DNA methyltransferase associated gene as the top-ranked result this seems a very useful area of research). I was a little underwhelmed by the chapter on diagnosis written by a retired New Zealand GP - it's not terrible; there are some good aspects - the lists of exclusionary conditions look reasonable, it mentions delayed PEM (albeit phrased as "post-exertional symptom flare-ups"), it recommends the Canadian criteria, but on the other hand good information is interspersed with the muddled in places.
(Mods may wish to move this to the second post in this thread) Most of these chapters have been posted in individual threads, so that in-place comments can be made by those with access or in reference to subsequent studies. Thread listing in no particular order — Profiling Antibody Reactivity to Gut Microbes in ME/CFS Patients Deep Immunophenotyping in ME/CFS Using Spectral Flow Cytometry Review of Neuroimaging Methods in ME/CFS Using the Ratio of Phosphorylated to Non-phosphorylated Forms of Stress Kinase PKR as a Potential Diagnostic Test for ME/CFS Quantitative Proteomics on Immune Cells of ME/CFS Patients Using SWATH-MS MicroRNA Profiling of Blood Extracellular Vesicles in ME/CFS Measurement of Genetic Variations in ME/CFS Patients in the IDO2 Gene Encoding an Enzyme Metabolizing Tryptophan Analysis of Transient Receptor Potential Ion Channels in ME/CFS Stress-Induced Changes in Immune Signatures in ME/CFS Patients Determined by Transcriptome Analysis Repeated Cardiopulmonary Exercise Testing of ME/CFS Patients Measuring Biomarkers of Oxidative Stress in ME/CFS Patients Mitochondrial Measures in Primary Cells Isolated from Patients with ME/CFS Application of DNA Methylome Analysis to Patients with ME/CFS Using Single-Cell Raman Microspectroscopy to Profile Human Peripheral Blood Mononuclear Cells Real-Time Measurement of Mitochondrial Function and Glycolysis in Lymphoblastoid Cell Lines
Should the initial post be pinned or tagged or whatever, so it's easily found by a researcher who's joined the forum to look for resources/pointers?
I can see this is a useful resource, and a great deal of work and thought has gone into it. But is it comprehensive? For example is there anything on genome analysis?
