Deep Immunophenotyping in ME/CFS Using Spectral Flow Cytometry, 2025, Gibson, Brooks et al.

Deep Immunophenotyping in ME/CFS Using Spectral Flow Cytometry
Gibson, Anton; Chometon, Thaize Q.; Damani, Tanvi; Brooks, Anna E. S.

Immune dysfunction is reported to play a significant role in the etiology of myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS). To gain an understanding of the underlying immune abnormalities associated with this complex condition, a comprehensive approach for characterizing immune cell subsets and their inferred functional states is essential.

We developed a high-dimensional flow cytometry method that enables detailed immunophenotyping of peripheral blood mononuclear cells (PBMCs) from ME/CFS patients. By simultaneously measuring over 40 markers on individual cells within one sample, this approach provides a comprehensive assessment of immune cell subsets, incorporating effector or functional states, to enable assessment of their potential roles in disease pathogenesis.

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Nice to see this from the New Zealand ME/CFS researcher, Anna Brooks, as an indication of her research focus.