De Abreu, Amirova, et al: Multi-System Deconditioning in 3-Day Dry Immersion without Daily Raise (microgravity simulation study)

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Multi-System Deconditioning in 3-Day Dry Immersion without Daily Raise

Abstract:
Dry immersion (DI) is a Russian-developed, ground-based model to study the physiological effects of microgravity. It accurately reproduces environmental conditions of weightlessness, such as enhanced physical inactivity, suppression of hydrostatic pressure and supportlessness.

We aimed to study the integrative physiological responses to a 3-day strict DI protocol in 12 healthy men, and to assess the extent of multi-system deconditioning. We recorded general clinical data, biological data and evaluated body fluid changes.

Cardiovascular deconditioning was evaluated using orthostatic tolerance tests (Lower Body Negative Pressure + tilt and progressive tilt). Metabolic state was tested with oral glucose tolerance test. Muscular deconditioning was assessed via muscle tone measurement.

Results: Orthostatic tolerance time dropped from 27 ± 1 to 9 ± 2 min after DI. Significant impairment in glucose tolerance was observed. Net insulin response increased by 72 ± 23% on the third day of DI compared to baseline. Global leg muscle tone was approximately 10% reduced under immersion.

Day-night changes in temperature, heart rate and blood pressure were preserved on the third day of DI. Day-night variations of urinary K+diminished, beginning at the second day of immersion, while 24-h K+ excretion remained stable throughout. Urinary cortisol and melatonin metabolite increased with DI, although within normal limits. A positive correlation was observed between lumbar pain intensity, estimated on the second day of DI, and mean 24-h urinary cortisol under DI.

In conclusion
, DI represents an accurate and rapid model of gravitational deconditioning. The extent of glucose tolerance impairment may be linked to constant enhanced muscle inactivity. Muscle tone reduction may reflect the reaction of postural muscles to withdrawal of support. Relatively modest increases in cortisol suggest that DI induces a moderate stress effect.

In prospect
, this advanced ground-based model is extremely suited to test countermeasures for microgravity-induced deconditioning and physical inactivity-related pathologies.


Steven De Abreu1†, Liubov Amirova1,2†, Ronan Murphy3, Robert Wallace3, Laura Twomey3, Guillemette Gauquelin-Koch4, Veronique Raverot5, Françoise Larcher6, Marc-Antoine Custaud1,7* and Nastassia Navasiolava7
  • 1Mitovasc, UMR Institut National de la Santé et de la Recherche Médicale 1083, Centre National de la Recherche Scientifique 6015, Université d'Angers, Angers, France
  • 2Russian Federation State Research Center, Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia
  • 3Center for Preventive Medicine, School of Health and Human Performance, Dublin City University, Dublin, Ireland
  • 4Centre National d'Etudes Spatiales, Paris, France
  • 5Hospices Civils de Lyon, Lyon, France
  • 6Laboratoire de Biochimie, Centre Hospitalier Universitaire d'Angers, Angers, France
  • 7Centre de Recherche Clinique, Centre Hospitalier Universitaire d'Angers, Angers, France
Front. Physiol., 13 October 2017 | https://doi.org/10.3389/fphys.2017.00799

Full text available
 
A total of twelve healthy non-athletic men aged 26 to 39 year. o. (age 32 ± 1.4 yr, weight 75 ± 2 kg, height 178 ± 2 cm, BMI 23.6 ± 0.4 kg/m2, maximal oxygen uptake VO2max 39 ± 1.1 mL/min/kg) were included in the study.

Subjects had no history of cardiovascular or other chronic diseases, and were not taking medication prior to the experiment. All subjects were informed about the experimental procedures and gave their written consent.

The experimental protocol conformed to the standards set by the Declaration of Helsinki and was approved by the local Ethic Committee (CPP Sud-Ouest Outre-Mer I, France) and French Health Authorities (n° ID RCB: 2014-A 00904-43).
 
Data are shown in Table 1. Water intake set ad libitum was decreased as anticipated on DI1, due to the known reset of water balance to lower level (Navasiolava et al., 2011c), but also on DI2 and DI3. This was likely due to discomfort during subject urination in the water tank, causing a voluntary reduction of water intake.

Blood volume decreased by 11% on R0 (from 6.45 ± 0.20 L to 5.74 ± 0.17 L, p < 0.001), while total Hb mass diminished by 3% (from 887 ± 33 g to 861 ± 33 g, p = 0.009). In relation to plasma volume, the CO technique showed a 16 ± 2% decrease at DI3 compared to DI1, and the Dill and Costill estimation showed a 14 ± 2% decrease vs. B-3. Hence, there was no significant difference between these two types of measurements. There were no changes in plasma volume at R+1 compared to baseline.

Pre-immersion plasma volume expressed in ml/kg correlated with initial VO2max (Pearson r = 0.67; p = 0.017), and percent reduction in plasma volume also correlated with initial VO2max (Pearson r = −0.76; p = 0.006), i.e., fitter subjects had greater relative plasma volume at baseline and greater hypovolemia under DI.
Be sure to expand that second quote
 
“Muscle tone variations under DI are shown in Figure 7. Global leg muscle tone, proxy measured by “frequency” parameter, was decreased by approximately 10% under immersion. This decrease was immediate (seen 6 h following the onset of DI) ...

“Six hours following the end of DI, muscle tone was completely restored.”
 
“Data are detailed in Table 2. Plasma electrolytes showed modest changes without clinical relevance.

“Blood count remained unchanged except for a modest but highly significant decrease in RBC volume, accompanied by slight increase in MCHC on R+1 vs. B-3.

“Blood GGT, ALAT, ASAT, alkaline phosphatase, total bilirubin, prothrombin time and serum albumin were not modified on R+1 vs. B-3. CRP levels were unaltered by DI.”
 
“Seventy-five percent of our subjects were intolerant to orthostasis on R0 after 3 days of strict DI. In comparison with literature data using chi-square test, orthostatic intolerance rate after strict bed rest without countermeasures does not differ significantly from our findings...

“However, the reported rate of orthostatic intolerance following non-strict DI seems much less-approximately 15 to 40% of subjects for 1 to 7 days of DI....

“The main differentiator of our DI was the maintenance of supine position throughout. Our data suggest that daily short periods out of the water tank in sitting or standing positions represent a powerful countermeasure against orthostatic intolerance induced by DI. Periodic short gravitational stimuli appear to be effective countermeasures, maintaining gravitational tolerance....

“Thus, a daily short period of orthostatic stress is sufficient to reduce the risk of orthostatic intolerance after simulated weightlessness. Daily short term gravitational load might also promote the preservation of baroreflex sensitivity and autonomic balance.”
 
“The negative metabolic effects of DI are mainly related to increased inactivity. DI rapidly impaired glucose metabolism and lipid profile, inducing a decrease in insulin sensitivity and dyslipidemia.”
 
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