Search results

gene.iobio highlights 4 moderate to high impact heterozygous rare variants I have on gene SLC25A5 but Genvue doesn't list them (must filter out for some reason?). I find having 4 a bit suspicious. They are on the X chromosome and I only have 1 X. EDIT : I checked one of the genes on gnomAD...

A friend came across this paper a few years ago. Ethanol metabolism: The good, the bad, and the ugly We found this section interesting. "Evidence supports the view that, following ethanol ingestion, elevated blood acetate becomes a major energy source for resting and moderately active muscle...

There seems to be a small subset that experience this at times. The majority report getting worse. I'd love to know why it helps. Thanks for the heads up on the analysis. I've only really used gene.iobio phenoanalyzer and an older hg37 custom tool..

Supplementary table S1 seems to have some sort of extra data but I'm not sure what it is listing. When analysing GWAS you need to be aware of miscalls for the platform you are using. I'm aware of some studies finding very high frequency vs low MAF or vice versa in cases and it turned out to be...

I can't make any sense of the data in table 1. How on earth do they get such low p-values for very common gene variants. Doesn't make sense to my simple understanding. I spot checked a few NBPF* variants in section A of table 1 and the internet genetic databases shows quite a lot of MAF...

There is another tool you can use to analyse VCF files, written by someone with ME/CFS. https://genvue.geneticgenie.org/ Some thoughts: * Variant frequency is important to look at and understand. Focus on the lower frequency ones to start with. * Clinvar is useful to see if the variant is known...

Thought I'd quote @Jonathan Edwards post from 1 year ago. What are the authors thoughts on the "junk antibodies" coming from B cell maturation changes in ME/CFS? Is there any evidence for something similar occurring in other diseases? Could there be an experiment to test if this is indeed...

These are the two threads for the wearable upright sensor work at the Bateman Horne Center Pilot study thread : Accurate and Objective Determination of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome Disease Severity with a Wearable Sensor Thesis thread : System and methods to determine...

The Thesis has moved to a preprint site hosted by Cornell https://arxiv.org/abs/2404.04345

A researcher in collaboration with BHC did use a commercial sensor worn on the lower leg with custom software and together they published a pilot study. Maybe someone can find a thread here with the details. The researcher was hoping to refine the software but I don't know what happened to that...

Thank you for taking the time to respond in such detail. In 2020 I took part in a very small unpublished pilot study that found "low level" connective tissue disease antibodies (sjogrens, lupus, dermatomyositis etc) vs healthy controls using a modern ELISA like array in a research lab. I think...

I think a clinician's view of ME/CFS is much broader than a researchers. Clinicians treat co-existing illnesses, some of which may have inflammation as defined by Jonathon. I think it's pretty clear by now that people throw the term "inflammation" about a lot but everyone seems to have a...

I should have added that Jonathon hinted there would be a paper coming regarding antibodies. He also hinted we should reread the hypothesis when DecodeME is published. Perhaps there is something else. I'm hoping when we get some additional evidence we will see some more meat in this hypothesis...

I think we have let a lot slide in this paper as it is a hypothesis paper. This seems to be the beans. 1. What evidence do we have for any "junk antibodies" in ME/CFS. I can only think of the Lipkin 1999 noting antibodies behave strange in ME/CFS. Perhaps his later B cell paper as well... 2...

This is from the transcript at the end of the webinar

I find it fascinating that lymphoblasts used in the work derive from B cells given the fact that Chris Armstrong & Mensah (Jo Cambridge lab) found energy issues in young B cells that caused changes in maturation. And now we have the Edwards, Cambridge, Cliff hypothesis of "junk" antibodies...

I've updated the link. Thanks for pointing out.

ME Group Australia YouTube Channel posted a talk with @DMissa "Understanding ME: Investigating cellular and body-wide features of ME" Dr Daniel Missailidis, PhD, discusses his work over the last 10 years in a very easy to understand way. The biggest finding to date from this team has been...

Comment on previous work on muscle cells + plasma at Oxford. They felt complement in plasma had a masking effect.

I'd just like to point out that this study was a replication of an experiment in Fluge et al paper of a decade ago. In simple terms it was an experiment to incubate some purchased model muscle cell samples with 20% PwME or HC serum. The Seahorse analyser has some very specific protocols on the...