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What jumps out at me from this is that the "MES test does not have the reliability and reproducibility required of a diagnostic test" & "The differences observed by the Myhill group may be down to differences in sample processing time between cohorts". So the test was unreliable; I assume that...

I tagged @Andy @Tom Kindlon @Simon M really just to highlight the reasons why ME is not funded i.e. not as a request that you lobby via ME Action. You run a good ship here on Science 4 ME - I've recommended this site to people taking refuge from the mother ship (Phoenix). I've been reviewed...

Karl Morten, in a presentation in New Zealand (2018? - you tube + thread on this site?) emphasised that samples are changing from the moment they are taken e.g. glucose is being consumed --. At the time I thought OK you've made your point Karl, move on; however, this paper sheds new light on his...

@Andy @Tom Kindlon @Simon M One of the interesting things which came up in discussions was why ME is not funded: there have been no specific research calls for ME issued by the European Union (EU) Commission. This is ring fenced funding i.e. it can only be allocated to the specified disease...

Unfortunately I have to agree i.e. about the influence of ME Community in the EU. Lyme disease has received 33.9 million euros in the last 10 years (development of a diagnostic test/treatments) from the European Commission; ME has received nothing. Lyme effects an estimated 1 million people in...

Unfortunately you do have to be a citizen of the EU to sign it. Thanks.

A friend on mine (Evelien) has succeeded in getting a petition on ME accepted by the EU Parliament [link below*]. The petition highlights the impact of ME on 2 million people in the EU and the lack of EU funding for ME Research. I'd be grateful if you would consider supporting it (and ask...

Looks interesting. We "know" that the signalling is coming from cells which are producing exosomes i.e. presumably something in the exosomes is causing the change in cellular energy production (from glucose to amino acids) and mitochondrial fragmentation. Looking at the proteins may give a clue...

Your post reminds me of something I'd picked up on recently - not 100% related to your question! I have a limited science training (as a technician - haven't worked in science for 15+ years). I think I've picked up that you need to ensure that your diagnostic method correctly identifies people...

I think Ron has spoken on a number of occasions about the decision to study the most severely ill patients (study design) - the logic is that this will provided a clearer insight into the disease compared with moderately ill people (my words). I wouldn't be entirely surprised to find that the...

I understand that it's the same as the tryptophan trap. I.e. 1) there's a substrate limited enzyme which functions at lower (tryptophan) levels; and 2) a second enzyme which operates at higher (tryptophan) levels. So if you have a non-functioning copy of the second enzyme then you can't...

I think there'll be a bunch of people who have altered metabolism caused by exosome signalling and others who have another disease (potentially known but undiagnosed - NIH study etc.). If this is correct then the data will clean up once a diagnostic test is applied. Yes everyone is important -...

Speculation! I don't necessarily think this is a problem. Another way to maintain a stable disease state [i.e. where cells switch from using glucose, for energy production, to amino acids] would be to maintain the input signal - i.e. the exosome signalling. The fact that those with a defective...

Really only stating the obvious here; I agree with your comment. I.e. the genetic data would need to be from people who tested positive using a biomedical diagnostic test e.g. the Raman spectroscopy test which measures (high) intracellular phenylalanine (Kara Morten, Cara Thomas and others) or...

Thanks to Andy I was able to access the paper. Here's the statement I was looking for

Yea this link doesn't work anymore - comes up with some (to me) indecipherable script. Thanks anyway.

Hi, can someone provide a link to the full paper? I think I read that this method is suitable for automation i.e. routine diagnostic testing. However, I can't find the reference - it may be in the full paper. Thanks.

I had a further look at Bhupesh Prusty's presentation at last months NIH Conference [https://videocast.nih.gov/summary.asp?Live=31640&bhcp=1 (starts around 3 hours 5 minutes)]. Bhupesh demonstrated fragmentation of mitocondria in ME. One of Bhupesh's colleagues at the University of Wuerzburg...

One of the things which have been on my mind about this is that initially the "something in the blood" which was effecting cellular energy production in ME was labelled as possibly an autoantibody. I posted that if it were an autoantibody then rituximab would have worked; however, rituximab...

Ron Davis highlighted that the best results from the nano-needle came from samples [peripheral blood monocytes] tested within 5 hours of drawing the blood. Karl Morten said the same (from memory) in New Zealand talk re ME/CFS plasma tested on muscle cells. I think this (5 hour window) also...