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Check out Ron Davis's presentation at the recent OMF Symposium [https://www.facebook.com/search/top/?q=part%202%3A%20community%20symposium%20on%20the%20molecular%20basis%20of%20me%2Fcfs&epa=SEARCH_BOX]. Indole propionate (a neuroprotectant) is made by a bacteria (Clostridium) in your gut...

I need to watch the videos again. I'm wondering if Ron's plasma swap experiment indicates that this is not something created by the storage conditions (using this anticoagulant or that)? E.g. plasma from controls (healthy people) didn't test positive on the nano-needle but plasma from people...

I'm wondering if a larger study on mitochondrial fragmentation (check out Bhupesh Prusty's presentation at NIH Conference April 2019) might provide some clarity. I.e. is the "something in the blood" a real thing or just an effect of different sample preparation techniques? If it is just a sample...

The Commission has previously "owned up" to not provided any specific funding for ME research "To date, no specific projects on ME/CFS have been supported by the EU Framework Programmes for Research and Innovation" [response to parliamentary question - December 2017 -...

Thank you very much.

Can you view this online i.e. after the date? Or are there minutes?

The first specific reference that I'm aware of was (December 2016) in this paper by Øystein Fluge and Karl Tronstad "Metabolic profiling indicates impaired pyruvate dehydrogenase function in myalgic encephalopathy/chronic fatigue syndrome" [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5161229/]...

Will the interview be available to view live and if so where & when? If not then will it be available to view later? Thanks.

No, your question is not naive --- not until we know the answer (which may be some time). I'm not medical either but Fluge and Mella published this statement "According to this model, ME/CFS is caused by immune interference with an unidentified target, potentially a signaling factor, which...

At one level the problem is simple i.e. because we know some things. E.g. if you review Karl Morten's work then you can see that healthy muscle cells + exosome (fraction) from ME plasma = a change in oxygen consumption (energy production) compared to plasma from healthy people. From this the...

@Andy Check out Karl Johan Tronstad's presentation at the Invest in ME Conference (May 2019 - http://www.investinme.org/IIMEC14.shtml). From about 11 minutes on he discusses dis-regulation of pyruvate dehydrogenase i.e. glucose cannot be used in the citric acid cycle for energy production -...

@Andy @Jonathan Edwards I watched Maureen Hanson's presentation today - i.e. presentation at the Invest in ME Conference (May 2019 - http://www.investinme.org/IIMEC14.shtml). If I picked it up correctly, they are finding larger numbers of exosomes i.e. in the plasma of people with ME compared to...

Yes i.e. I think I recall that normal oxygen concentration for muscle cells is 8% and ambient (air) is 22% - so presumably they "incubate" at 8% oxygen for the experiment. Also, the key point is that the healthy muscle cells, with ME plasma, are consuming more oxygen --- "red line is lower...

@Andy @wigglethemouse may have provided an answer to the question - i.e. can the assessment of oxygen consumption be used as a diagnostic test? I.e. no since the baseline is too noisy (data above shows a wide range in the measured values) - possibly Karl's slide in the presentation shows...

I assume that @wigglethemouse has provided the answer i.e. you add inhibitors and then commence the measurements. Presumably it takes a short period of time before the system stabilises. I'm not terribly concerned to see; however, the answer may be interesting - if I can understand it! One of...

Firstly the red dots are the results from a plasma sample, from someone with ME, added to healthy muscle cells. The key thing is that the cells are consuming oxygen more quickly but are not necessarily producing energy efficiently (check out @wigglethemouse post/links to sections of the talk -...

I'd like to see some data i.e. from the NIH study and possibly from Julia Newton's group at Newcastle University. How many people tested positive using something like Karl's oxygen consumption test/nano-needle i.e. have this reversible change in cellular energy production? How many people were...

@Andy Q1: Can the change in oxygen consumption, i.e. when ME plasma added to muscle cells versus normal plasma, be used to diagnose ME? [see graph - courtesy of @wigglethemouse ] Currently people with ME do not know if they have this "abnormality" [change in oxygen consumption/nano-needle...

Can anyone explain why this cannot be developed ointo a diagnostic test i.e. "He did a plasma swap experiment with healthy muscle cells and showed oxygen consumption is different when mixed with patient plasma vs healthy plasma"? @Andy @JaimeS

Without wishing to be alarmist here there are reasons why laboratories are regulated e.g. Industrial Bio-Test Laboratories [https://en.wikipedia.org/wiki/Industrial_Bio-Test_Laboratories]. Things "go wrong" for various reasons --- we need regulators. If you wish to see a model test then check...