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Probably, I can't recall anybody else measuring 4EBP1 and he mentioned "lymphoblasts". There is a terminology problem here, though. The lab I worked in at the time was a microbiology lab that had adopted human cell culture recently. So what we now call LCLs (and are commonly called LCLs) we used...

You have kind of spear gunned the elephant in the room here. It's been a bit of a source of puzzlement for me to see, in some cases, pathway-specific biology applied to disease models without a heap of specific tissue or cell type rationale or little reference to current genetic evidence. It is...

at a glance the IL-10 and IL-6 results seem interesting in terms of HC PCC ME differences

I just went through 7 different gene expression datasets that I have for different cell types from people with ME/CFS and nothing for these two genes stood out as clearly altered or consistent in direction of change from a quick scan. I was hoping to see something because they could fit well...

we have not measured a ratio of Ca2+ to SOD2

This was also what I saw in the cell lines

Dont have time to go thru it but are medications factored in? could affect the properties of circulating cells what will be interesting to me is to see how this looks vs other conditions

I was going to comment that the literature summary is factually incorrect in several areas… but then I saw “AI” was used… :/

I have a methodological comment relating to the original 2016 seahorse design (the current study is a replication effort so it is not a knock against the current study) in the seahorse done originally in the 2016 paper, the injection were glucose -> oligomycin -> fccp ->rotenone+antimycin A. I...

Yep, it is very variable. The important thing you can do is perform the assay with a great deal of care and consistency, many replicates within the same experiments (we aim for at least 5 or 6, this is standard) and do at least 3 runs of each sample (we'll often do many more). The team here...

vaccines causing autism was retracted and look where we are at today. memeability is hard to beat

I will minimise the OT but it is a Pratchett character :)

This title is going to have a big effect when authors of other papers go google searching for anything related to relationships between ME/CFS and LC. It's going to float to the top of those google searches and get taken at face value and plugged into endnote in a heartbeat. It scarcely even...

By chance I was thinking about the Scotchgard thing last night (I was at the conference and it stuck in my brain) and I went back to that slide just now and the fungicide afecting b-ox is interesting. ~35 mins if interested

Murph, since you have taken at interest in this, keep an eye out for probably my next paper re cholesterol

The point is that of the however many areas likely needed to understand what's going on, inflammation is one of them (because the word is still being used, and knowing whether it is founded on firm grounding or not is important) and all I am seeking is a bit of context to help steer me through...

Well for example what I asked about was the inflammation/Klimas issue and the response which boiled down to "the other person doesn't understand this" didn't give me much to go on. edit: I now see another more recent reply about what was in the paper: This would have been a good way to avoid...

I think it would be more productive to address inconsistencies or instances of alleged misunderstanding directly, point by point. It may not matter how obvious things might seem if they aren’t communicated. We like to dismiss a lot of things as “handwaving” but we need to also hold ourselves to...

I am not a psychologist. I could not understand a lot of the conclusions and psychology of the Wallitt paper which is why I have never really commented about it. It was over my head. I understood this paper, start-to-finish, perfectly on a first pass. Really nice work making this accessible.

Our lab is exploring this area