Search results

Yep, I will be. It's not final and needs a lot more analysis, and is only a small update on a small branch of the projects I'm working on, but I hope it's at least interesting. Looking at combined metabolomes and lipidomes from whole cells using the lymphoblastoid cell lines. Am under embargo...

I think I probably phrased things poorly in a hurry to post that between tasks in the lab. You're of course correct. What I mean to say is that I think LC represents potentially either, or both of, the following: 1) Something similar to, or potentially the same as, ME/CFS, but with a different...

"Autoimmunity is considered as a key causal factor for diseases like ME/CFS." Is it? I didn't know that we figured out what's causing the disease yet. About the literature: "Chronic post viral illnesses like ME/CFS and long COVID affect multiple body systems and may lead to development of...

Perhaps the authors felt that the outcomes of thousands of concurrent measurements could not be fairly represented or even-handedly conveyed by only including a small selection of detailed specifics in as limited a space as an abstract or a conclusion. Seems reasonable to me if so.

The kinetics of transient activation by immunological stimulus are different between individuals. And yes this extends to cells in vitro, we have seen it in the lab. Without a time-course that captures the curve for each individual it's therefore hard to know what to make of results obtained at...

Cerebral hypoperfusion causes vascular dementia. Do we know if VaD and ME/CFS are frequently present together? I haven't encountered it, but the clinical side of things is not where I spend most of my time.

This is pretty technical. We're on the same page. :) This is a very good example of why you can't have too much detail in a methods section. For all we know they've done this but not explained it. But it's absolutely necessary to apply any meaning to the outcome!!

It looks like they've applied whole exosomes, not just DNA to the cells, so this part should be unaffected by that issue. (The raw result anyway), any further interpretations that the response is due to the mtDNA specifically would to me seem unfounded due to the DNA quantification issue and due...

Did they? Without a standard curve in the qPCR or without a total DNA quantification assay, I can't see how they'd know this. The qPCR, as shown, can only indicate the 7S content in the pooled ME/CFS samples relative to the pooled control samples, irrespective of total DNA abundance. So it's not...

I don't see how this is publishable unless I have missed something. I do not see mention of a DNA loading control/housekeeping gene or any quantification of loaded DNA. For all we know there are different amounts of total DNA in each sample. Unless this is clarified to be an oversight in the...

First, awesome paper and glad to see the Morten lab getting their ME/CFS work into the literature. Very nice to see other people looking to cell-based biomarker potential. This matter is a bit complicated. It's kind of a replication but also not. There are similarities and differences in both...

Did anybody catch if the exercise event durations for each individual or clinical group were included in the paper? Can't see it at the moment. Approx 8-10 min seems to be the typical length of the exertion period but would be good to know what the difference in mechanical performance during the...

Unless there is a specific reason for this concern, it could be applied to any scientific observation. Of course, as a reader this is always borne in mind with individual or not yet validated studies, but when you see the same trend across multiple sample types, investigative techniques...

This is cool, in line with what prior work has been suggesting and from a new angle (new cell types, more direct measures of function). I love this lab's work. Immune cells from pwme, in vitro, are definitely seeming to want more fats based on what we can see in the current literature. I really...

Assumption here being that CSF carries the underlying signature of both diseases.

Hey Simon, really good point. Sorry for the extreme delay in seeing this. Yep, true. I am increasingly thinking that the way to approach these wide-net -omics studies (for things that aren't DNA - ie: metabolomics, proteomics) is to focus on them as exploratory grounds for hypothesis generation...

Hey everybody. Sorry for the absence, have been overwhelmed with work. This discussion is important and don't worry about scaring me away or anything. I have raised the concerns herein with my colleagues with a mind to avoid this in the future. I hear you all. Also, regarding the discussion...

Thank you, Hutan. I am glad to take part in whichever way is appropriate and productive. The driver for why we work in this field should always be community centred... wanting people to get better. I try to osmose this mentality to new students. I will raise this tomorrow. I think there is a...

Thanks Snowy. I can always count on you for this kind of useful scrutiny. And yes, you are right - there is no intrinsic reason for a link. Multi-system epigenetic changes could be one explanation if a phenotypic pattern is present across multiple body systems/tissue types. Currently pursuing...

Re the discussion of glutamine metabolism, glucose, fats (etc) in prior work: indeed, nothing is proven yet. Good news is that people are doing follow-up studies to validate these theories (including with different methods or with limitations of prior studies in mind). Some of these projects...