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https://www.google.com/search?q=mbl+deficiency+prevalence&rlz=1C1ONGR_en-GBAU995AU995&oq=mbl+deficiency+prevalence&aqs=chrome..69i57.275j0j1&sourceid=chrome&ie=UTF-8

EVs are a field very much in vogue at the moment so expect a lot more in this direction is my guess

Generally speaking yes but depends on specifics of: 1) is the signal being measured detectable in the other particular cell type. Eg: mito function troublesome to measure in quiescent cells 2) utility of doing it depends on whether the pathology is tissue- or cell type-specific (don't know...

Okay, I have answers! Yay. 1) Aspects of fibroblast cell culture that I raised appear to be handled sensibly, carefully, thoroughly and attentively. I am pleased with the information provided by email. Amazing. 2) Protein assay involved lysis (from email) which negates potential small concerns...

Paul Hwang sent me a couple of lovely responses, seems like a really nice guy. I didn't get the full picture because he's out of the office and my questions are very technically detailed. I may get more info later from another author. When I have concrete info I may share here if appropriate -...

@chillier thanks for asking for detail and holding my statements accountable. Should actually happen more. Density (contact inhibition): I don't know about published seahorse studies off the top of my head but I have seen it firsthand in the lab with seahorse, and others have demonstrated it...

I see that we may be having similar friendly conversations away from s4me :D very exciting To add my own experience: we did not see an effect of freezing duration on changes in viability (data in our biomarker paper), but didn't check for effects of freezing duration for the few seahorse...

I think of it as: DNA is the blueprint, mRNA is a photocopy of the blueprint sent to the factory floor, protein is what the photocopy tells the assembly line (ribosome) to make. As for the paper: "Seahorse XF Metabolic Studies. The cells were trypsinized, resuspended in Seahorse XF DMEM assay...

I do, and I agree and am currently playing with it, but the information is sadly incomplete because we don't have access to the patient records of CFS Discovery since it closed. But yes, with the clinical/patient information we do have, we look for relationships with clusters, whenever clusters...

Interesting. The lipidomics I presented at IACFSME 2023 showed broadly elevated levels of lipids in lymphoblastoid cell lines generated from pwME.

I really hope this is an English issue more than somebody walking in and just defining the disease into their existing research box.

The bottom line with prospective markers is that nothing has been properly revisited and validated in publication since the wave of modern biomarker studies began a handful of years ago. We have many papers finding lab differences that can separate already defined clinical groups. That's only...

You don't need to qualify your questions before asking :). Thanks for engaging in such a great way with your thoughts and questions. There are a few probable reasons for differences between studies and generally speaking I would say that they are not necessarily contradictory (at least I think...

I want to look at muscle but it's just been so practically challenging. Getting enough material to do the extent of in-depth experiments that I do (with muscle biopsies) from a sufficient number of sedentary controls in addition to patients.. is a huge problem. (sedentary controls are absolutely...

Thanks for the link to relevant discussion. I went down the rabbit hole from your link, and saw some s4me chatter about the unlikelihood that a direct ATP deficiency is at play. TBH I am probably inclined to agree if you ask me that question today, as a guess only... my thinking +...

Thank you for the clarification @Jonathan Edwards, I think I misconstrued one or two elements of your post. I agree with your frustrations regarding overuse of the word and obsession over classification accuracy without due consideration of either practicability or true clinical utility. In...

Not mutually exclusive. Also, yes, if a biological feature being used as a diagnostic is one that's related to the underlying causal mechanism, it is probably more likely to work, in principle. Of course. However, does that notion then preclude other features that are consequences, not causes...

Whole blood mito material will be affected by muscle damage due to physical activity, was physical activity prior to sampling, per individual, quantified in some way and assessed for relationship with the other measurements? Currently jet-lagged so might be missing it. Experienced clinicians...

Sorry that i saw this so late, I don't keep good track of my s4me notifications and rarely have time for a thorough browse. My recollection was that fluge/mella did non-cellular metabolomics (serum or plasma, whichever one) and PBMC RNA measurements to complement their ideas. I don't think...

Very compelling ideas. I really can't wait to meet her in a week or so at IACFSME. I think she's at the forefront of the field right now. Very high quality work and intelligent direction.