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In strongly agreeing with this I would also add as a general comment to readers that I think tissue context always needs to be front and centre when we think about these metabolic questions. Eg a T cell upregulating glycolysis or a muscle cell doing so can be driven by mechanisms not even...

If I am reading this right their ME/CFS data is the RNAseq data from the Wallitt study muscle samples. DNA -> RNA -> protein -> metabolic function. This paper seems to say: RNA = metabolic function = treatment target. Am I missing something? "GPMM 10 integrates protein abundance estimates...

My guess is regulatory disarray. Something in metabolism / signalling going wrong that has wide ranging knock-on effects that include changes to mitochondria

ay, there’s the rub edit: Hi Chris, I didn't realise this was you. No wonder you were making so much sense. HAHAHA

Posting a paper here: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6690029/ A good resource for those who want context needed to understand just what these cell lines actually are or might mean when studied. I basically didn't address a lot of this in my early years (papers or thesis). In a...

Will discuss with the person we settle on :)

If anybody knows someone very experienced and composed at taking blood in VIC, please send them my way. We need a phlebotomist for the severe pwME home visits. I want someone really good so that things go smoothly for our participants. (Our previous people have moved on). They will be paid for...

Thanks Hutan, that confirms my gut suspicions but I wasn’t certain enough to comment. Good to know.

"Using flow cytometry, we compared mitochondrial bioenergetic parameters in CD19 B cells as well as CD4 and CD8 T cell lymphocyte populations. Sample gating strategy for identifying these lymphocyte populations is shown in Supplementary Figure S1A. Although we did not identify any differences...

We have intentionally excluded family in the past so trust me I understand. We will exclude family members with known history of similar or relevant conditions (including POTS, we will never treat anybody with POTS as a healthy control). I anticipate that we will have very few if any family...

Hadn't thought of this, I will look into it, thank you. On another note, if anybody has family or friends who may be interested as healthy controls please put them in touch @ D.Missailidis@latrobe.edu.au . Similarly if anybody with access to closed pwME networks could share this message it...

Making sense of gut data is very difficult due to the number of influencing factors so work like this is extremely important.

I only wish that there was a way to access those too severe to use the internet beyond lucking out with their carers. Thank you everybody else for your support and spreading it, it has shown in huge spikes in responses since I woke up this morning. It really helps and matters a lot. Especially...

I am absolutely shocked by how many clinicians I have met who treat anecdotal information with less skepticism than published studies or trials.

Hi everybody, I finally have the ethics in place to begin recruiting for our recently funded Mason Foundation study. ICC criteria. Healthy controls and pwME in VIC, Australia. Involves some questionaires and a blood and feces collection. We will home visit severely affected people. Primary...

Such studies would happen more with better funding!!!

Happy to report that upon another look this paper seems to replicate closely with my own work I just checked the unadjusted* mito red fluorescence of about 100 ME/CFS LCLs and something like 60 or 70 HC and it is also reduced as seen in the B cells in this paper. Sharing it here because that...

LDs are important in the innate immune response so differences in their dynamics in a pathogen context make sense. If there are LD dynamics differences in pwME immune tissues/cells (for example) it could be one of two things happening: 1) an underlying difference in immune response leads to...

there's also this thread that I saw years ago: https://www.s4me.info/threads/lipid-droplets-have-they-been-investigated-in-me-should-they-be.17424/#post-296600 funnily enough one of the authors of the papers referenced in there sits a few meters from me and we are working together on the side...

Hi people. Some due context. We have crazy smart friends over in chemistry who make things like these cool and novel dyes. This paper is focused on one such dye. They wanted a demonstration of potential disease context applications and we had suspicions about LDs so we ran a small pilot sample...