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:) Of that we can only dream. (oh, apart from DecodeME)

Some background information about RER, it's probably over-simplifying things: https://web.cortland.edu/buckenmeyerp/fall2004/labrvalue.html This is the machine that reports the RER and oxygen use and CO2 production in everyday settings: I agree that the concept of this study was excellent...

I wonder how many of these 23 studies had suitable controls. Studies without active controls can't tell us if these interventions (in populations where the majority will be improving with time) are of any use. And yet:

One of the authors is Sarah Tyson @sarahtyson. The assessments were done in 2021, and the study found that even people with severe ME/CFS were mostly able to use monitoring equipment without problems. I'm therefore not understanding Sarah's conclusion, expressed on the forum earlier this year...

and all of the usual misinformed easy opinions about ME and FND

Yes. Just to reiterate this, here's some of the text from the webpage I linked to upthread that I thought set it out well: Not that I'm suggesting that the authors here were trying to suggest more about their data than was warranted. It's just - please give us the scatter plots. That's...

Also, with the recent WASF3 study in mind, we need a similar sort of study on the effect of standard processing and storage on other types of cells.

So, keeping storage times to an absolute minimum, reducing variation in this and detailed reporting. Also, EDTA tubes were recommended. I think this study raises some important questions for biobanks. I think they need to be very selective about what sort of analyses they supply stored blood...

Thanks @Andy, this is a really important issue for all of the studies that have been done using the Seahorse analyser. We've been calling for information on this. The paper describes the Seahorse analysis in a readable way and gives clear definitions of the parameters reported It is good to...

(I like your avatar @chillier, is that just new? Nudibranchs are glorious.)

Going a bit further on in the paper WASF3 Suppresses Mitochondrial Respiration So, this is still with the cells of patient S1. The expression of the WASF3 gene was reduced (using shRNA - a short hairpinRNA). The authors say that this The higher black line is the increased oxygen consumption...

My response to that cannot be written here. But it really shows that these people have no idea what they are talking about. Relax? Tired? I know we've said it all before, but it's the sheer arrogance these people have, to think that we have not tried to ignore the symptoms and keep going...

Were the participants required to exercise in the metabolic room?

I had to search to find out what the error bars are for Figure 5b (that's the WASF3 levels from the NIH ME/CFS study participants). I eventually found a reference to one of the charts in the Supplementary Information to Standard Error. In my experience, standard errors on charts are not a good...

Thanks for that @DMissa I've only read a bit of the paper so far. The sedentary comparison is certainly something I've been thinking about, indeed it could easily be a confounder. There is a reference to the NIH ME/CFS study in the paper, with respect to the WASF3 findings: I was expecting...

The researchers compared fibroblasts of S1 with her brother S2, who also carried a P38 mutation. They found that S1's fibroblasts had lower oxygen consumption rate (OCR) and decreased Complex IV (one of the electron transport complexes in the mitochondrial membrane). Complex IV has two...

Thanks @B_V S1 (Subject 1) had a history of worsening fatigue after mononucleosis at age 16 and two separate early onset breast cancers, successfully treated. She had frequent cramps in her lower leg muscles at rest and exercise intolerance that required days to recover from exertion. The...

This paper could be worth a look too, in this case alpha-synuclein is in the serum. I'm not sure if it is phosphorylated. Elevated ATG13 in serum of pwME stimulates oxidative stress response in microglial cells , 2022, Gottschalk et al

From the NICE Guideline

There is an overview of autonomic dysfunction that makes the following comment: The grand challenge of autonomic disorders It gives 5 references that may be of interest for people wanting to look into this further.