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Platelet Storage Pool Deficiency and Elevated Inflammatory Biomarkers Are Prevalent in POTS, 2022, Gunning, Grubb et al

Discussion in ''Conditions related to ME/CFS' news and research' started by Ryan31337, Feb 23, 2022.

  1. Ryan31337

    Ryan31337 Senior Member (Voting Rights)

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    Platelet Storage Pool Deficiency and Elevated Inflammatory Biomarkers Are Prevalent in Postural Orthostatic Tachycardia Syndrome
    by William T. Gunning, Paula M. Kramer, Jacob A. Cichock, Beverly L. Karabin, Sadik A. Khuder, Blair P. Grubb
    doi.org/10.3390/cells11050774

    https://www.mdpi.com/2073-4409/11/5/774

    Abstract
    A significant number of postural orthostatic tachycardia syndrome (POTS) patients have platelet delta granule storage pool deficiency (δ-SPD). The etiology of POTS is unknown but a number of laboratories, including ours, have reported elevations of G-protein-coupled adrenergic receptor and muscarinic acetylcholine receptor autoantibodies in POTS patients, detected by a variety of techniques, suggesting that the disorder is an autoimmune condition. Thus, it could also be considered an inflammatory disease. In a pilot study, we investigated a limited number of platelet-related cytokines and chemokines and discovered many that were elevated. This case–control study validates our pilot study results that POTS patients have an activated innate immune system.

    Plasma of 35 POTS patients and 35 patients with unexplained bleeding symptoms and categorized as “non-POTS” subjects was analyzed by multiplex flow cytometry to quantify 16 different innate immune system cytokines and chemokines. Electron microscopy was used to quantify platelet dense granules. Ten of 16 biomarkers of inflammation were elevated in plasma from POTS patients compared to non-POTS subjects, with most of the differences extremely significant, with p values < 0.0001. Of particular interest were elevations of IL-1β and IL-18 and decreased or normal levels of type 1 interferons in POTS patients, suggesting that the etiology of POTS might be autoinflammatory. All POTS patients had δ-SPD.

    With a growing body of evidence that POTS is an autoimmune disease and having elevations of the innate immune system, our results suggest a potential T-cell-mediated autoimmunity in POTS characteristic of a mixed-pattern inflammatory disease similar to rheumatoid arthritis.

    Full text, open access: https://www.mdpi.com/2073-4409/11/5/774/pdf
     
    Hutan, jaded, livinglighter and 8 others like this.
  2. Ryan31337

    Ryan31337 Senior Member (Voting Rights)

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    From this paper:

    upload_2022-2-23_23-38-54.png

    From the earlier pilot study:
    [​IMG]

    The assumption seems to be that the delta granule storage pool deficiency being seen in such high numbers is acquired and a result of chronic inflammation.
     
    Last edited: Feb 24, 2022
  3. Jonathan Edwards

    Jonathan Edwards Senior Member (Voting Rights)

    Messages:
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    Location:
    London, UK
    If they had put the numerical data in the abstract rather than talking immunological gibberish I would have taken more note. I got as far in the methods as 'retrospective case control study'.

    Forget it.

    Cytokines can cause inflammation when produced OUTSIDE the circulation - to call cells out into the tissues. Cytokines in the circulation cannot do that so it is hard to know what they mean - unless you have already seen some inflammation from which this might be spillover. Since nobody has seen any inflammation in people with POTS as far as I know the whole thing is meaningless.
     
    alktipping, CRG, FMMM1 and 2 others like this.
  4. Ryan31337

    Ryan31337 Senior Member (Voting Rights)

    Messages:
    359
    Consequence of COVID shutdown unfortunately. Prospective study now underway.
     
    alktipping and SNT Gatchaman like this.

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